What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Activity and specificity of TRV-mediated gene editing in plants

Typ / Jahr

Journal Article / 2015


Ali, Zahir; Abul-faraj, Aala; Piatek, Marek; Mahfouz, Magdy M.


Plant trait engineering requires efficient targeted genome-editing technologies. Clustered regularly interspaced palindromic repeats (CRISPRs)/ CRISPR associated (Cas) type II system is used for targeted genome-editing applications across eukaryotic species including plants. Delivery of genome engineering reagents and recovery of mutants remain challenging tasks for in planta applications. Recently, we reported the development of Tobacco rattle virus (TRV)-mediated genome editing in Nicotiana benthamiana. TRV infects the growing points and possesses small genome size; which facilitate cloning, multiplexing, and agroinfections. Here, we report on the persistent activity and specificity of the TRV-mediated CRISPR/Cas9 system for targeted modification of the Nicotiana benthamiana genome. Our data reveal the persistence of the TRV- mediated Cas9 activity for up to 30 d post-agroinefection. Further, our data indicate that TRV-mediated genome editing exhibited no off-target activities at potential off-targets indicating the precision of the system for plant genome engineering. Taken together, our data establish the feasibility and exciting possibilities of using virus-mediated CRISPR/Cas9 for targeted engineering of plant genomes.

Clustered Regularly Interspaced Short Palindromic Repeats; DNA, Plant; Genes, Plant; Genetic Engineering/methods; Genome, Plant; Mutagenesis, Site-Directed; Mutation; Plant viruses; RNA editing; RNA Viruses; RNA, Viral; Tobacco/genetics/virology
Plant signaling & behavior
Periodical Number
Page range


ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
6 Magdy M Mahfouz
Saudi Arabia
Nicotiana benthamiana CRISPR/Cas9
Albino and dwarf phenotype
Basic research
Basic research