Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Agrobacterium-mediated Gene Transformation of Cas9 into Flammulina velutipes

495

Typ / Jahr

Journal Article / 2018

Autoren

LIU Jianyu, LIU Jianhui, ZHANG Dan, ZHANG Meiyan, XU Zhen, WANG Ruijuan, YU Hailong, SHANG Xiaodong

Abstract

The nucleotide sequence of Cas9 gene from Streptococcus pyogenes (SpCas9, Addgene # 43802) was codon-optimized according to the codon usage bias of Flammulina velutipes. An optimized FvCas9 sequence was generated, and then used to construct binary expression plasmid FpiC. The plasmid was then transformed into one of F. velutipes monokaryon 'Dan3' mediated by A. tumefaciens. Positive transformants were initially selected by hygromycin resistance and then confirmedby PCR detection of the hygromycin resistant gene (hyg) and FvCas9 gene on the Fpic vector. The PCR results showed that both hyg and FvCas9 gene were successfully inserted into the genome of F. velutipes, with a transformation rate of 6,84%.

Keywords

Agrobacterium-mediated transformation; Cas9; CRISPR/Cas9 system; Flammulina velutipes

Periodical

Periodical Number

Page range

Volume

DOI

10.16488/j.enki.1005

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Techniques

ID	Corresponding Author Country	Plant Species	GE Technique Sequence Identifier	Trait Type of Alteration	Progress in Research Key Topic
1236	Shang, Xiaodong China	Flammulina velutipes	CRISPR/Cas9 No information	No information SDN1	Basic research Basic research