Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

479

Typ / Jahr

Journal Article / 2017

Autoren

Shimatani, Zenpei; Kashojiya, Sachiko; Takayama, Mariko; Terada, Rie; Arazoe, Takayuki; Ishii, Hisaki; Teramura, Hiroshi; Yamamoto, Tsuyoshi; Komatsu, Hiroki; Miura, Kenji; Ezura, Hiroshi; Nishida, Keiji; Ariizumi, Tohru; Kondo, Akihiko

Abstract

We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.

Keywords

Periodical

Nature biotechnology

Periodical Number

Page range

441–443

Volume

DOI

10.1038/nbt.3833

View Publication

Techniques

ID	Corresponding Author Country	Plant Species	GE Technique Sequence Identifier	Trait Type of Alteration	Progress in Research Key Topic
1178	Ezura, Hiroshi; Nishida, Keiji; Ariizumi, Tohru Japan	Oryza sativa	Base-editing ALS	Herbicide tolerance BE	Basic research Basic research
1179	Ezura, Hiroshi; Nishida, Keiji; Ariizumi, Tohru Japan	Oryza sativa	Base-editing FTIP1e	Herbicide tolerance BE	Basic research Basic research
1180	Ezura, Hiroshi; Nishida, Keiji; Ariizumi, Tohru Japan	Oryza sativa	Base-editing ALS	Herbicide tolerance BE	Market-oriented Herbicide tolerance
1181	Ezura, Hiroshi; Nishida, Keiji; Ariizumi, Tohru Japan	Solanum lycopersicum	Base-editing DELLA	Regulation of plant hormone signaling BE	Basic research Basic research
1182	Ezura, Hiroshi; Nishida, Keiji; Ariizumi, Tohru Japan	Solanum lycopersicum	Base-editing ETR1	Regulation of plant hormone signaling BE	Basic research Basic research