Repositorium
Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice
476
Journal Article / 2017
Shen, Lan; Hua, Yufeng; Fu, Yaping; Li, Jian; Liu, Qing; Jiao, Xiaozhen; Xin, Gaowei; Wang, Junjie; Wang, Xingchun; Yan, Changjie; Wang, Kejian
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated endonuclease 9 (CRISPR/Cas9) system has emerged as a promising technology for specific genome editing in many species. Here we constructed one vector targeting eight agronomic genes in rice using the CRISPR/Cas9 multiplex genome editing system. By subsequent genetic transformation and DNA sequencing, we found that the eight target genes have high mutation efficiencies in the T0 generation. Both heterozygous and homozygous mutations of all editing genes were obtained in T0 plants. In addition, homozygous sextuple, septuple, and octuple mutants were identified. As the abundant genotypes in T0 transgenic plants, various phenotypes related to the editing genes were observed. The findings demonstrate the potential of the CRISPR/Cas9 system for rapid introduction of genetic diversity during crop breeding.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
1165 |
Yan, Changjie; Wang, Kejian China |
Oryza sativa |
CRISPR/Cas9 DEP1 |
Dense and erect panicle SDN1 |
Market-oriented Agronomic value |
1166 |
Yan, Changjie; Wang, Kejian China |
Oryza sativa |
CRISPR/Cas9 Gn1a |
Grain number SDN1 |
Market-oriented Agronomic value |
1167 |
Yan, Changjie; Wang, Kejian China |
Oryza sativa |
CRISPR/Cas9 GS3 |
Grain length and weight SDN1 |
Market-oriented Agronomic value |
1168 |
Yan, Changjie; Wang, Kejian China |
Oryza sativa |
CRISPR/Cas9 GW2 |
Grain width and weight SDN1 |
Market-oriented Agronomic value |
1169 |
Yan, Changjie; Wang, Kejian China |
Oryza sativa |
CRISPR/Cas9 LPA1 |
Loose plant architecture SDN1 |
Market-oriented Agronomic value |
1170 |
Yan, Changjie; Wang, Kejian China |
Oryza sativa |
CRISPR/Cas9 BADH2 |
Fragrant genotype SDN1 |
Market-oriented Product quality |
1171 |
Yan, Changjie; Wang, Kejian China |
Oryza sativa |
CRISPR/Cas9 EP3 |
Erect panicle SDN1 |
Basic research Basic research |
1172 |
Yan, Changjie; Wang, Kejian China |
Oryza sativa |
CRISPR/Cas9 Hd1 |
Heading date SDN1 |
Basic research Basic research |