Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

High efficiency of targeted mutagenesis in arabidopsis via meiotic promoter-driven expression of Cas9 endonuclease

47

Typ / Jahr

Journal Article / 2016

Autoren

Eid, Ayman; Ali, Zahir; Mahfouz, Magdy M.

Abstract

The use of a meiosis I-specific promoter increased the efficiency of targeted mutagenesis and will facilitate the manipulation of homologous recombination. The CRISPR/Cas9 system has been harnessed for targeted engineering of eukaryotic genomes, including plants; however, CRISPR/Cas9 efficiency varies considerably in different plant tissues and species. In Arabidopsis, the generation of homozygous or bi-allelic mutants in the first (T1) generation is inefficient. Here, we used specific promoters to drive the expression of Cas9 during meiosis to maximize the efficiency of recovering heritable mutants in T1 plants. Our data reveal that the use of a promoter active in meiosis I resulted in high-efficiency (28 %) recovery of targeted mutants in the T1 generation. Moreover, this method enabled efficient simultaneous targeting of three genes for mutagenesis. Taken together, our results show that the use of meiosis-specific promoters will improve methods for functional genomic analysis and studying the molecular underpinnings of homologous recombination.

Keywords

Arabidopsis Proteins/genetics; Arabidopsis/genetics; Base Sequence; CRISPR/Cas9 Genome engineering Genome editing Meiotic promoters Targeted mutagenesis Arabidopsis; CRISPR-Cas Systems; Endonucleases/genetics/metabolism; Gene Editing/methods; Genetic Engineering/methods; homologous recombination; homozygote; Meiosis/genetics; Models, Genetic; Mutagenesis, Site-Directed/methods; Mutation; Plants, Genetically Modified; Promoter Regions, Genetic/genetics; Reproducibility of Results

Periodical

Plant cell reports

Periodical Number

Page range

1555–1558

Volume

DOI

10.1007/s00299-016-2000-4

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Techniques

ID	Corresponding Author Country	Plant Species	GE Technique Sequence Identifier	Trait Type of Alteration	Progress in Research Key Topic
88	Mahfouz, Magdy M. Saudi Arabia	Arabidopsis thaliana	CRISPR/Cas9 CPC	No information SDN1	Basic research Basic research
89	Mahfouz, Magdy M. Saudi Arabia	Arabidopsis thaliana	CRISPR/Cas9 ETC2	Altered leaf phenotype SDN1	Basic research Basic research
90	Mahfouz, Magdy M. Saudi Arabia	Arabidopsis thaliana	CRISPR/Cas9 TRY	curled leafes SDN1	Basic research Basic research