Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato


Typ / Jahr

Journal Article / 2017

Autoren

Mahfouz, Magdy; Tashkandi, Manal; Ali, Zahir; Aljedaani, Fatimah; Shami, Ashwag

Abstract

CRISPR/Cas systems confer molecular immunity against phages and conjugative plasmids in prokaryotes. Recently, CRISPR/Cas9 systems have been used to confer interference against eukaryotic viruses. Here, we engineered Nicotiana benthamiana and tomato (Solanum lycopersicum) plants with the CRISPR/Cas9 system to confer immunity against the Tomato yellow leaf curl virus (TYLCV). Targeting the TYLCV genome with Cas9-single guide RNA at the sequences encoding the coat protein (CP) or replicase (Rep) resulted in efficient virus interference, as evidenced by low accumulation of the TYLCV DNA genome in the transgenic plants. The CRISPR/Cas9-based immunity remained active across multiple generations in the N. benthamiana and tomato plants. Together, our results confirmed the efficiency of the CRISPR/Cas9 system for stable engineering of TYLCV resistance in N. benthamiana and tomato, and opens the possibilities of engineering virus resistance against single and multiple infectious viruses in other crops.

Keywords
CRISPR/Cas9 system; molecular immunity; tomato (Solanum lycopersicum); tomato transformation and regeneration.; Tomato yellow leaf curl virus (TYLCV); virus resistance
Periodical
Periodical Number
Page range
Volume
DOI
10.1101/237735

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
1036 Mahfouz, Magdy M.
Saudi Arabia
Nicotiana benthamiana CRISPR/Cas9
TYLCV
Immunity against the Tomato yellow leaf curl virus
SDN1
Basic research
Basic research
1037 Mahfouz, Magdy M.
Saudi Arabia
Solanum lycopersicum CRISPR/Cas9
TYLCV
Immunity against the Tomato yellow leaf curl virus
SDN1
Market-oriented
Biotic stress tolerance