Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Efficient targeted mutagenesis in soybean by TALENs and CRISPR/Cas9


Typ / Jahr

Journal Article / 2016

Autoren

Du, Hongyang; Zeng, Xuanrui; Zhao, Meng; Cui, Xiaopei; Wang, Qing; Yang, Hui; Cheng, Hao; Yu, Deyue

Abstract

Gene targeting (GT) is of great significance for advancing basic plant research and crop improvement. Both TALENs (transcription activator-like effectors nucleases) and CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated 9) systems have been developed for genome editing in eukaryotes, including crop plants. In this work, we present the comparative analysis of these two technologies for two soybean genome editing targets, GmPDS11 and GmPDS18. We found GT in soybean hairy roots with a single targeting efficiency range of 17.5–21.1% by TALENs, 11.7–18.1% by CRISPR/Cas9 using the AtU6-26 promoter, and 43.4–48.1% by CRISPR/Cas9 using the GmU6-16g-1 promoter, suggesting that the CRISPR/Cas9 using the GmU6-16g-1 promoter is probably a much more efficient tool compared to the other technologies. Similarly, our double mutation GT efficiency experiment with these three technologies displayed a targeting efficiency of 6.25% by TALENs, 12.5% by CRISPR/Cas9 using the AtU6-26 promoter, and 43.4–48.1% by CRISPR/Cas9 using the GmU6-16g-1 promoter, suggesting that CRISPR/Cas9 is still a better choice for simultaneous editing of multiple homoeoalleles. Furthermore, we observed albino and dwarf buds (PDS knock-out) by soybean transformation in cotyledon nodes. Our results demonstrated that both TALENs and CRISPR/Cas9 systems are powerful tools for soybean genome editing.

Keywords
Base Sequence; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR/Cas9; CRISPR-Cas Systems; Gene Knockdown Techniques; Genetic Engineering/methods; Genetic Vectors; genome editing; Molecular Sequence Data; Mutagenesis, Insertional/methods; Oxidoreductases/genetics/metabolism; Plants, Genetically Modified; RNA editing; Sequence Deletion; soybean; Soybean Proteins/genetics/metabolism; Soybeans/genetics/growth & development; TALENs
Periodical
Journal of biotechnology
Periodical Number
Page range
90–97
Volume
217
DOI
10.1016/j.jbiotec.2015.11.005

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
83 Yu, Deyue
China
Glycine Max CRISPR/Cas9
PDS11
Albino phenotype
SDN1
Basic research
Basic research
84 Yu, Deyue
China
Glycine Max CRISPR/Cas9
PDS18
Albino phenotype
SDN1
Basic research
Basic research
85 Yu, Deyue
China
Glycine Max TALENs
PDS11
Albino and dwarf phenotype
SDN1
Basic research
Basic research
86 Yu, Deyue
China
Glycine Max TALENs
PDS18
Albino and dwarf phenotype
SDN1
Basic research
Basic research