Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Efficient targeted mutagenesis in soybean by TALENs and CRISPR/Cas9

45

Typ / Jahr

Journal Article / 2016

Autoren

Du, Hongyang; Zeng, Xuanrui; Zhao, Meng; Cui, Xiaopei; Wang, Qing; Yang, Hui; Cheng, Hao; Yu, Deyue

Abstract

Gene targeting (GT) is of great significance for advancing basic plant research and crop improvement. Both TALENs (transcription activator-like effectors nucleases) and CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated 9) systems have been developed for genome editing in eukaryotes, including crop plants. In this work, we present the comparative analysis of these two technologies for two soybean genome editing targets, GmPDS11 and GmPDS18. We found GT in soybean hairy roots with a single targeting efficiency range of 17.5–21.1% by TALENs, 11.7–18.1% by CRISPR/Cas9 using the AtU6-26 promoter, and 43.4–48.1% by CRISPR/Cas9 using the GmU6-16g-1 promoter, suggesting that the CRISPR/Cas9 using the GmU6-16g-1 promoter is probably a much more efficient tool compared to the other technologies. Similarly, our double mutation GT efficiency experiment with these three technologies displayed a targeting efficiency of 6.25% by TALENs, 12.5% by CRISPR/Cas9 using the AtU6-26 promoter, and 43.4–48.1% by CRISPR/Cas9 using the GmU6-16g-1 promoter, suggesting that CRISPR/Cas9 is still a better choice for simultaneous editing of multiple homoeoalleles. Furthermore, we observed albino and dwarf buds (PDS knock-out) by soybean transformation in cotyledon nodes. Our results demonstrated that both TALENs and CRISPR/Cas9 systems are powerful tools for soybean genome editing.

Keywords

Base Sequence; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR/Cas9; CRISPR-Cas Systems; Gene Knockdown Techniques; Genetic Engineering/methods; Genetic Vectors; genome editing; Molecular Sequence Data; Mutagenesis, Insertional/methods; Oxidoreductases/genetics/metabolism; Plants, Genetically Modified; RNA editing; Sequence Deletion; soybean; Soybean Proteins/genetics/metabolism; Soybeans/genetics/growth & development; TALENs

Periodical

Journal of biotechnology

Periodical Number

Page range

90–97

Volume

217

DOI

10.1016/j.jbiotec.2015.11.005

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Techniques

ID	Corresponding Author Country	Plant Species	GE Technique Sequence Identifier	Trait Type of Alteration	Progress in Research Key Topic
83	Yu, Deyue China	Glycine Max	CRISPR/Cas9 PDS11	Albino phenotype SDN1	Basic research Basic research
84	Yu, Deyue China	Glycine Max	CRISPR/Cas9 PDS18	Albino phenotype SDN1	Basic research Basic research
85	Yu, Deyue China	Glycine Max	TALENs PDS11	Albino and dwarf phenotype SDN1	Basic research Basic research
86	Yu, Deyue China	Glycine Max	TALENs PDS18	Albino and dwarf phenotype SDN1	Basic research Basic research