Repositorium
Typ / Jahr
Journal Article / 2018
Autoren
Zou, Ting; Liu, Mingxing; Xiao, Qiao; Wang, Tao; Chen, Dan; Luo, Tao; Yuan, Guoqiang; Li, Qiao; Zhu, Jun; Liang, Yueyang; Deng, Qiming; Wang, Shiquan; Zheng, Aiping; Wang, Lingxia; Li, Ping; Li, Shuangcheng
Abstract
Key message OsPKS2, the rice orthologous gene of Arabidopsis PKSB/LAP5, encodes a polyketide synthase that is involved in pollen wall formation in rice.
Abstract In flowering plants, the pollen wall protects male gametes from various environmental stresses and pathogen attacks, as well as promotes pollen germination. The biosynthesis of sporopollenin in tapetal cell is critical for pollen wall formation. Recently, progress has been made in understanding sporopollenin metabolism during pollen wall development in Arabidopsis. However, little is known about the molecular mechanism that underlies the sporopollenin synthesis in pollen wall formation in rice (Oryza sativa). In this study, we identified that a point mutation in OsPKS2, a plant-specific type III polyketide synthase gene, caused male sterility in rice by affecting the normal progress of pollen wall formation. Two other allelic mutants of OsPKS2 were generated using the CRISPR/Cas9 system and are also completely male sterile. This result thus further confirmed that OsPKS2 controls rice male fertility. We also showed that OsPKS2 is an orthologous gene of Arabidopsis PKSB/LAP5 and has a tapetum-specific expression pattern. In addition, its product localizes in the endoplasmic reticulum. Results suggested that OsPKS2 is critical for pollen wall formation, and plays a conserved but differentiated role in sporopollenin biosynthesis from Arabidopsis.
Keywords
Exine; OsPKS2; PKSB/LAP5; Pollen; rice; Sporopollenin
Periodical
Plant cell reports
Periodical Number
5
Page range
759–773
Volume
37
DOI
10.1007/s00299-018-2265-x
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
923 |
Li, Ping; Li, Shuangcheng China |
Oryza sativa |
CRISPR/Cas9 PKS2 |
Male fertility SDN1 |
Basic research Basic research |