Repositorium
Typ / Jahr
Journal Article / 2017
Autoren
Zou, Ting; He, Zhiyuan; Qu, Lingyun; Liu, Mingxing; Zeng, Jing; Liang, Yanling; Wang, Tao; Chen, Dan; Xiao, Qiao; Zhu, Jun; Liang, Yueyang; Deng, Qiming; Wang, Shiquan; Zheng, Aiping; Wang, Lingxia; Li, Ping; Li, Shuangcheng
Abstract
Pollen development in flowering plants is critical for male reproductive success. The pollen wall that protects the pollen from various environment stresses and bacterial infections plays an essential role in pollen development. The formation of pollen wall is associated with the biosynthesis and transport ofsporopollenin components. ACOS5 in Arabidopsis encodes an acyl-CoA synthetase 5 required for sporopollenin biosynthesis. We identified the rice homolog ofACOS5 as OsACOS12. The CRISPR/Cas9-mediated OsACOS12 knockout mutant has complete male sterility due to a defect in pollen wall formation. β- Glucuronidase reporter gene analysis and RNA in situ hybridization indicated that OsACOS12 was specifically expressed in tapetum and microspores. The subcellular localization of OsACOS12-YFP demonstrated that OsACOS12 protein was primarily localized in the endoplasmic reticulum and nucleus. Our results suggest that OsACOS12 plays a critical and conserved role in pollen wall formation and pollen development and has implications in rice breeding.
Keywords
ACOS5; Arabidopsis; male sterility; OsACOS12; rice
Periodical
Mol Breeding (Molecular Breeding)
Periodical Number
10
Page range
437
Volume
37
DOI
10.1007/s11032-017-0722-9
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
922 |
Li, Ping; Li, Shuangcheng China |
Oryza sativa |
CRISPR/Cas9 ACOS12 |
Male sterility SDN1 |
Market-oriented Agronomic value |