Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Knockout of OsACOS12 caused male sterility in rice


Typ / Jahr

Journal Article / 2017

Autoren

Zou, Ting; He, Zhiyuan; Qu, Lingyun; Liu, Mingxing; Zeng, Jing; Liang, Yanling; Wang, Tao; Chen, Dan; Xiao, Qiao; Zhu, Jun; Liang, Yueyang; Deng, Qiming; Wang, Shiquan; Zheng, Aiping; Wang, Lingxia; Li, Ping; Li, Shuangcheng

Abstract
Pollen development in flowering plants is critical for male reproductive success. The pollen wall that protects the pollen from various environment stresses and bacterial infections plays an essential role in pollen development. The formation of pollen wall is associated with the biosynthesis and transport ofsporopollenin components. ACOS5 in Arabidopsis encodes an acyl-CoA synthetase 5 required for sporopollenin biosynthesis. We identified the rice homolog ofACOS5 as OsACOS12. The CRISPR/Cas9-mediated OsACOS12 knockout mutant has complete male sterility due to a defect in pollen wall formation. β- Glucuronidase reporter gene analysis and RNA in situ hybridization indicated that OsACOS12 was specifically expressed in tapetum and microspores. The subcellular localization of OsACOS12-YFP demonstrated that OsACOS12 protein was primarily localized in the endoplasmic reticulum and nucleus. Our results suggest that OsACOS12 plays a critical and conserved role in pollen wall formation and pollen development and has implications in rice breeding.
Keywords
ACOS5; Arabidopsis; male sterility; OsACOS12; rice
Periodical
Mol Breeding (Molecular Breeding)
Periodical Number
10
Page range
437
Volume
37
DOI
10.1007/s11032-017-0722-9

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
922 Li, Ping; Li, Shuangcheng
China
Oryza sativa CRISPR/Cas9
ACOS12
Male sterility
SDN1
Market-oriented
Agronomic value