Repositorium
Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance
bearbeiten
399
Typ / Jahr
Journal Article / 2018
Autoren
Zhou, Xiaogang; Liao, Haicheng; Chern, Mawsheng; Yin, Junjie; Chen, Yufei; Wang, Jianping; Zhu, Xiaobo; Chen, Zhixiong; Yuan, Can; Zhao, Wen; Wang, Jing; Li, Weitao; He, Min; Ma, Bingtian; Wang, Jichun; Qin, Peng; Chen, Weilan; Wang, Yuping; Liu, Jiali; Qian, Yangwen; Wang, Wenming; Wu, Xianjun; Li, Ping; Zhu, Lihuang; Li, Shigui; Ronald, Pamela C.; Chen, Xuewei
Abstract
Crops carrying broad-spectrum resistance loci provide an effective strategy for controlling infectious disease because these loci typically confer resistance to diverse races of a pathogen or even multiple species of pathogens. Despite their importance, only a few crop broad-spectrum resistance loci have been reported. Here, we report the identification and characterization of the rice bsr-k1 (broadspectrum resistance Kitaake-1) mutant, which confers broad-spectrum resistance against Magnaporthe oryzae and Xanthomonas oryzae pv oryzae with no major penalty on key agronomic traits. Map-based cloning reveals that Bsr-k1 encodes a tetratricopeptide repeats (TPRs)containing protein, which binds to mRNAs of multiple OsPAL (OsPAL1– 7) genes and promotes their turnover. Loss of function of the Bsr-k1 gene leads to accumulation of OsPAL1–7 mRNAs in the bsr-k1 mutant. Furthermore, overexpression of OsPAL1 in wild-type rice TP309 confers resistance to M. oryzae, supporting the role of OsPAL1. Our discovery of the bsr-k1 allele constitutes a significant conceptual advancement and provides a valuable tool for breeding broad-spectrum resistant rice.
Keywords
blast disease; Broad-spectrum resistance; innate immunity; rice; TPR protein
Periodical
Proceedings of the National Academy of Sciences of the United States of America
Periodical Number
12
Page range
3174–3179
Volume
115
DOI
10.1073/pnas.1705927115
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
921 |
Chen, Xuewei China |
Oryza sativa |
CRISPR/Cas9 Os10g0548200 |
Resistance to bacterial blight SDN1 |
Market-oriented Biotic stress tolerance |