What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

CRISPR-Cas9 and CRISPR-Cpf1 mediated targeting of a stomatal developmental gene EPFL9 in rice

Typ / Jahr

Journal Article / 2017


Yin, Xiaojia; Biswal, Akshaya K.; Dionora, Jacqueline; Perdigon, Kristel M.; Balahadia, Christian P.; Mazumdar, Shamik; Chater, Caspar; Lin, Hsiang-Chun; Coe, Robert A.; Kretzschmar, Tobias; Gray, Julie E.; Quick, Paul W.; Bandyopadhyay, Anindya


16 Key Message: CRISPR-Cas9/Cpf1 system with its unique gene targeting efficiency, could be an important tool for functional study of early developmental genes through the generation of successful knockout plants. 2Cpf1 technology to knock out an early developmental gene EPFL9 (Epidermal Patterning Factor 5 Abstract The introduction and utilization of systems biology approaches have identified several genes that are involved in early development of a plant and with such knowledge a robust tool is required for the functional validation of putative candidate genes thus obtained. The development of the CRISPR-Cas9/Cpf1 genome editing system has provided a convenient tool for creating loss of function mutants for genes of interest. The present study utilized CRISPR/Cas9 and CRISPR-Cpf1 technology to knock out an early developmental gene EPFL9 (Epidermal Patterning Factor like-9,a positive regulator of stomatal development in Arabidopsis) orthologue in rice. Germline mutants that were generated showed edits that were carried forward into the T2 generation 2 when Cas9-free homozygous mutants were obtained. The homozygous mutant plants showed more than an eight-fold reduction in stomatal density on the abaxial leaf surface of the edited rice plants. otential off-target analysis showed no significant off-target effects. This study also utilized the CRISPR-LbCpf1 (Lachnospiracae bacterium Cpf1) to target the same OsEPFL9 gene to test the activity of this class-2 CRISPR system in rice and found that Cpf1 is also capable of genome editing and edits get transmitted through generations with similar phenotypic changes seen with CRISPR-Cas9. This study demonstrates the application of CRISPR-Cas9/Cpf1 to precisely target genomic locations and develop transgene-free homozygous heritable gene edits and confirms that the loss of function analysis of the candidate genes emerging from different systems biology based approaches, could be performed and therefore this system adds value in the validation of gene function studies.

Plant cell reports
Periodical Number
Page range


ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
877 Bandyopadhyay, Anindya
Oryza sativa CRISPR/Cas9
Decreased stomatal density
Basic research
Basic research
878 Bandyopadhyay, Anindya
Oryza sativa CRISPR/Cpf1
Decreased stomatal density
Basic research
Basic research