Repositorium
Journal Article / 2018
Yang, Jungil; Luo, Dangping; Yang, Bing; Frommer, Wolf B.; Eom, Joon-Seob
Despite the relevance of seed-filling mechanisms for crop yield, we still have only a rudimentary understanding of the transport processes that supply the caryopsis with sugars. We hypothesized that SWEET sucrose transporters may play important roles in nutrient import pathways in the rice caryopsis. We used a combination of mRNA quantification, histochemical analyses, translational promoter–reporter fusions and analysis of knockout mutants created by genomic editing to evaluate the contribution of SWEET transporters to seed filling. In rice caryopses, SWEET11 and 15 had the highest mRNA levels and proteins localized to four key sites: all regions of the nucellus at early stages; the nucellar projection close to the dorsal vein; the nucellar epidermis that surrounds the endosperm; and the aleurone. ossweet11;15 double knockout lines accumulated starch in the pericarp, whereas caryopses did not contain a functional endosperm. Jointly, SWEET11 and 15 show all the hallmarks of being necessary for seed filling with sucrose efflux functions at the nucellar projection and a role in transfer across the nucellar epidermis/aleurone interface, delineating two major steps for apoplasmic seed filling, observations that are discussed in relation to observations made in rice and barley regarding the relative prevalence of these two potential import routes.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
866 |
Frommer, Wolf B.; Eom, Joon-Seab Germany; USA; Japan |
Oryza sativa |
CRISPR/Cas9 SWEET11 |
Essential for seed filling SDN1 |
Basic research Basic research |
867 |
Frommer, Wolf B.; Eom, Joon-Seab Germany; USA; Japan |
Oryza sativa |
TALENs SWEET11 |
Essential for seed filling SDN1 |
Basic research Basic research |
868 |
Frommer, Wolf B.; Eom, Joon-Seab Germany; USA; Japan |
Oryza sativa |
TALENs SWEET15 |
Essential for seed filling SDN1 |
Basic research Basic research |