Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

A Collection of Mutants for CLE-Peptide-Encoding Genes in Arabidopsis Generated by CRISPR/Cas9-Mediated Gene Targeting


Typ / Jahr

Journal Article / 2017

Autoren

Yamaguchi, Yasuka L.; Ishida, Takashi; Yoshimura, Mika; Imamura, Yuko; Shimaoka, Chie; Sawa, Shinichiro

Abstract

The ligand–receptor-mediated intercellular communication system plays important roles in coordinating developmental and physiological events in multicellular organisms. In plants, CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptides and their cognate receptors are thought to be involved in various aspects of the plant life cycle. Although the importance of this communication is broadly recognized, most CLE peptides are yet to be functionally characterized. A major problem in research on small signaling peptide-encoding genes is the limited number of loss-of-function mutants available due to their small gene size. CRISPR/Cas9-mediated gene targeting has the potential to overcome this problem, as it can be used to generate targeted mutations in essentially any gene, regardless of size. Here we generated a series ofmutants of CLE-peptide-encoding genes. Newly generated clv3 and cle40 mutants reproduced the expected mutant phenotypes in the shoot apical meristem and root meristem, respectively. Our results show that CRISPR/Cas9-mediated gene targeting is a powerful tool for genetic analyses, even of small genes. We also report a novel mutant for CLE44 [which is thought to encode a tracheary elements differentiation inhibitory factor (TDIF)] and show that CLE44 contributes to vascular development. The bioresources presented here will be a powerful tool for further characterization of CLE peptides.

Keywords
Cell-to-cell communication; CLE peptides; CRISPR/Cas9; loss of function; mutant collection.
Periodical
Plant & cell physiology
Periodical Number
11
Page range
1848–1856
Volume
58
DOI
10.1093/pcp/pcx139

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
854 Ishida, Takashi
Japan
Arabidopsis thaliana CRISPR/Cas9
CLE44
Contribution to vascular development
SDN1
Basic research
Basic research
855 Ishida, Takashi
Japan
Arabidopsis thaliana CRISPR/Cas9
CLV3
pleiotropic abnormalities in organs derived from the shoot apical meristem
SDN1
Basic research
Basic research