Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Role of heterotrimeric Gα proteins in maize development and enhancement of agronomic traits


Typ / Jahr

Journal Article / 2018

Autoren

Wu, Qingyu; Regan, Michael; Furukawa, Hiro; Jackson, David

Abstract

Plant shoot systems derive from the shoot apical meristems (SAMs), pools of stems cells that are regulated by a feedback between the WUSCHEL (WUS) homeobox protein and CLAVATA (CLV) peptides and receptors. The maize heterotrimeric G protein α subunit COMPACT PLANT2 (CT2) functions with CLV receptors to regulate meristem development. In addition to the sole canonical Gα CT2, maize also contains three eXtra Large GTP-binding proteins (XLGs), which have a domain with homology to Gα as well as additional domains. By either forcing CT2 to be constitutively active, or by depleting XLGs using CRISPR-Cas9, here we show that both CT2 and XLGs play important roles in maize meristem regulation, and their manipulation improved agronomic traits. For example, we show that expression of a constitutively active CT2 resulted in higher spikelet density and kernel row number, larger ear inflorescence meristems (IMs) and more upright leaves, all beneficial traits selected during maize improvement. Our findings suggest that both the canonical Gα, CT2 and the non-canonical XLGs play important roles in maize meristem regulation and further demonstrate that weak alleles of plant stem cell regulatory genes have the capacity to improve agronomic traits.

Keywords
Periodical
PLoS genetics
Periodical Number
4
Page range
Volume
14
DOI
10.1371/journal.pgen.1007374

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
849 Jackson, David
USA
Zea mays CRISPR/Cas9
XLG1
Lethality at the seedling stage
SDN1
Basic research
Basic research
850 Jackson, David
USA
Zea mays CRISPR/Cas9
XLG3a
Lethality at the seedling stage
SDN1
Basic research
Basic research
851 Jackson, David
USA
Zea mays CRISPR/Cas9
XLG3b
Lethality at the seedling stage
SDN1
Basic research
Basic research