Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Transgenerational CRISPR-Cas9 Activity Facilitates Multiplex Gene Editing in Allopolyploid Wheat


Typ / Jahr

Journal Article / 2018

Autoren

Wang, Wei; Pan, Qianli; He, Fei; Akhunova, Alina; Chao, Shiaoman; Trick, Harold; Akhunov, Eduard

Abstract

The CRISPR-Cas9-based multiplexed gene editing (MGE) provides a powerful method to modify multiple genomic regions simultaneously controlling different agronomic traits in crops. We applied the MGE construct built by combining the tandemly arrayed tRNA–gRNA units to generate heritable mutations in the TaGW2, TaLpx-1, and TaMLO genes of hexaploid wheat. The knockout mutations generated by this construct in all three homoeologous copies of one of the target genes, TaGW2, resulted in a substantial increase in seed size and thousand grain weight. We showed that the non-modified gRNA targets in the early generation plants can be edited by CRISPR-Cas9 in the following generations. Our results demonstrate that transgenerational gene editing activity can serve as the source of novel variation in the progeny of CRISPR-Cas9-expressing plants and suggest that the Cas9inducible trait transfer for crop improvement can be achieved by crossing the plants expressing the gene editing constructs with the lines of interest.

Keywords
Periodical
The CRISPR Journal
Periodical Number
1
Page range
65–74
Volume
1
DOI
10.1089/crispr.2017.0010

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
838 Akhunov, Eduard
USA
Triticum aestivum CRISPR/Cas9
GW2
Increase in seed size and thousand grain weight
SDN1
Market-oriented
Agronomic value
839 Akhunov, Eduard
USA
Triticum aestivum CRISPR/Cas9
Lpx-1
Resistance to Fusarium graminearum
SDN1
Basic research
Basic research
840 Akhunov, Eduard
USA
Triticum aestivum CRISPR/Cas9
MLO
Resistance against powdery mildew
SDN1
Basic research
Basic research