Repositorium
Transgenerational CRISPR-Cas9 Activity Facilitates Multiplex Gene Editing in Allopolyploid Wheat
358
Journal Article / 2018
Wang, Wei; Pan, Qianli; He, Fei; Akhunova, Alina; Chao, Shiaoman; Trick, Harold; Akhunov, Eduard
The CRISPR-Cas9-based multiplexed gene editing (MGE) provides a powerful method to modify multiple genomic regions simultaneously controlling different agronomic traits in crops. We applied the MGE construct built by combining the tandemly arrayed tRNA–gRNA units to generate heritable mutations in the TaGW2, TaLpx-1, and TaMLO genes of hexaploid wheat. The knockout mutations generated by this construct in all three homoeologous copies of one of the target genes, TaGW2, resulted in a substantial increase in seed size and thousand grain weight. We showed that the non-modified gRNA targets in the early generation plants can be edited by CRISPR-Cas9 in the following generations. Our results demonstrate that transgenerational gene editing activity can serve as the source of novel variation in the progeny of CRISPR-Cas9-expressing plants and suggest that the Cas9inducible trait transfer for crop improvement can be achieved by crossing the plants expressing the gene editing constructs with the lines of interest.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
838 |
Akhunov, Eduard USA |
Triticum aestivum |
CRISPR/Cas9 GW2 |
Increase in seed size and thousand grain weight SDN1 |
Market-oriented Agronomic value |
839 |
Akhunov, Eduard USA |
Triticum aestivum |
CRISPR/Cas9 Lpx-1 |
Resistance to Fusarium graminearum SDN1 |
Basic research Basic research |
840 |
Akhunov, Eduard USA |
Triticum aestivum |
CRISPR/Cas9 MLO |
Resistance against powdery mildew SDN1 |
Basic research Basic research |