What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Lipid productivity in TALEN-induced starchless mutants of the unicellular green alga Coccomyxa sp. strain Obi

Typ / Jahr

Journal Article / 2018


Takahashi, Keita; Ide, Yoko; Hayakawa, Jumpei; Yoshimitsu, Yuya; Fukuhara, Izumi; Abe, Jun; Kasai, Yuki; Harayama, Shigeaki


Starchless mutants of several green algae including Chlamydomonas reinhardtii and Scenedesmus obliquus showed improved lipid productivity. In this study, we isolated mutants of the unicellular green alga, Coccomyxa sp. strain Obi, defective in the gene for the large subunit of ATP:α-D-glucose-1-phosphate adenylyltransferase (AGPL)by means of a transcription activator-like effector nuclease (TALEN) as follows. First, two expression cassettes encoding left and right arms of TALEN for AGPL mutagenesis were introduced into strain Obi, and from two independent transformants of strain Obi carrying both expression cassettes, seven derivatives bearing different mutations in AGPL were isolated. When these mutants were grown in 1/2-diluted and 1/3-diluted minimal media, their lipid contents were significantly higher than those of their parental strains. The growth in 1/3diluted medium was similar between the mutants and their parental strains, while the growth in 1/2-diluted medium was significantly lower for the mutants than for their parental strains. The lipid productivity, which is a crucial factor for commercial production of biofuels, was not statistically different between the parental and mutant strains. We concluded that the suppression of starch biosynthesis to increase lipid productivity in microalgae is a risky strategy as it could result in low biomass productivity.

biofuel; Green alga; Starch; TALEN; α-D-Glucose-1-phosphate adenylyltransferase
Algal Research
Periodical Number
Page range


ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
822 Harayama, Shigeaki
Pseudococcomyxa ellipsoidea TALENs
Starchless phenotype
Basic research
Basic research