Repositorium
Journal Article / 2013
Christian, Michelle; Qi, Yiping; Zhang, Yong; Voytas, Daniel F.
Custom TAL effector nucleases (TALENs) are increasingly used as reagents to manipulate genomes in vivo. Here, we used TALENs to modify the genome of the model plant, Arabidopsis thaliana. We engineered seven TALENs targeting five Arabidopsis genes, namely ADH1, TT4, MAPKKK1, DSK2B, and NATA2. In pooled seedlings expressing the TALENs, we observed somatic mutagenesis frequencies ranging from 2-15% at the intended targets for all seven TALENs. Somatic mutagenesis frequencies as high as 41-73% were observed in individual transgenic plant lines expressing the TALENs. Additionally, a TALEN pair targeting a tandemly duplicated gene induced a 4.4-kb deletion in somatic cells. For the most active TALEN pairs, namely those targeting ADH1 and NATA2, we found that TALEN-induced mutations were transmitted to the next generation at frequencies of 1.5-12%. Our work demonstrates that TALENs are useful reagents for achieving targeted mutagenesis in this important plant model.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
59 |
Voytas, Daniel F. USA |
Arabidopsis thaliana |
TALENs ADH1 |
Bacteria stress tolerance - resistant to bacterial leaf blight SDN1 |
Basic research Basic research |
60 |
Voytas, Daniel F. USA |
Arabidopsis thaliana |
TALENs DSK2B |
Bacteria stress tolerance - resistant to bacterial leaf blight SDN1 |
Basic research Basic research |
61 |
Voytas, Daniel F. USA |
Arabidopsis thaliana |
TALENs GLL22 |
gene cluster gll22 SDN1 |
Basic research Basic research |
62 |
Voytas, Daniel F. USA |
Arabidopsis thaliana |
TALENs MAPKKK1 |
N.A. SDN1 |
Basic research Basic research |
63 |
Voytas, Daniel F. USA |
Arabidopsis thaliana |
TALENs NATA2 |
N.A. SDN1 |
Basic research Basic research |
64 |
Voytas, Daniel F. USA |
Arabidopsis thaliana |
TALENs TT4 |
transparent testa SDN1 |
Basic research Basic research |