Repositorium
The fungal pathogen Magnaporthe oryzae suppresses innate immunity by modulating a host potassium channel
346
Journal Article / 2018
Shi, Xuetao; Long, Yu; He, Feng; Zhang, Chongyang; Wang, Ruyi; Zhang, Ting; Wu, Wei; Hao, Zeyun; Wang, Yi; Wang, Guo-Liang; Ning, Yuese
Potassium (K+) is required by plants for growth and development, and also contributes to immunity against pathogens. However, it has not been established whether pathogens modulate host K+ signaling pathways to enhance virulence and subvert host immunity. Here, we show that the effector protein AvrPiz-t from the rice blast pathogen Magnaporthe oryzae targets a K+ channel to subvert plant immunity. AvrPiz-t interacts with the rice plasma-membrane-localized K+ channel protein OsAKT1 and specifically suppresses the OsAKT1mediated K+ currents. Genetic and phenotypic analyses show that loss of OsAKT1 leads to decreased K+ content and reduced resistance against M. oryzae. Strikingly, AvrPiz-t interferes with the association of OsAKT1 with its upstream regulator, the cytoplasmic kinase OsCIPK23, which also plays a positive role in K+ absorption and resistance to M. oryzae. Furthermore, we show a direct correlation between blast disease resistance and external K+ status in rice plants. Together, our data present a novel mechanism by which a pathogen suppresses plant host immunity by modulating a host K+ channel.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
813 |
Wang, Yi; Wang, Guo-Liang; Ning Yuese China; USA |
Oryza sativa |
CRISPR/Cas9 CIPK23 |
Reduced resistance to M.oyzae SDN1 |
Basic research Basic research |