What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

A farnesyl pyrophosphate synthase gene expressed in pollen functions in S -RNase-independent unilateral incompatibility

Typ / Jahr

Journal Article / 2018


Qin, Xiaoqiong; Li, Wentao; Liu, Yang; Tan, Meilian; Ganal, Martin; Chetelat, Roger T.


Multiple independent and overlapping pollen rejection pathways contribute to unilateral interspecific incompatibility (UI). In crosses between tomato species, pollen rejection usually occurs when the female parent is self-incompatible (SI) and the male parent self-compatible (SC) (the ‘SI 3 SC rule’). Additional, as yet unknown, UI mechanisms are independent of self-incompatibility and contribute to UI between SC species or populations. We identified a major quantitative trait locus on chromosome 10 (ui10.1) which affects pollen-side UI responses in crosses between cultivated tomato, Solanum lycopersicum, and Solanum pennellii LA0716, both of which are SC and lack S-RNase, the pistil determinant of S-specificity in Solanaceae. Here we show that ui10.1 is a farnesyl pyrophosphate synthase gene (FPS2) expressed in pollen. Expression is about 18-fold higher in pollen of S. pennellii than in S. lycopersicum. Pollen with the hypomorphic S. lycopersicum allele is selectively eliminated on pistils of the F1 hybrid, leading to transmission ratio distortion in the F2 progeny. CRISPR/Cas9-generated knockout mutants (fps2)in S. pennellii LA0716 are self-sterile due to pollen rejection, but mutant pollen is fully functional on pistils of S. lycopersicum.F2 progeny of S. lycopersicum 3 S. pennellii (fps2) show reversed transmission ratio distortion due to selective elimination of pollen bearing the knockout allele. Overexpression of FPS2 in S. lycopersicum pollen rescues the pollen elimination phenotype. FPS2-based pollen selectivity does not involve S-RNase and has not been previously linked to UI. Our results point to an entirely new mechanism of interspecific pollen rejection in plants.

farnesyl pyrophosphate synthase; num lycopersicum; Sola-; Solanum pennellii.; transmission ratio distortion; unilateral incompatibility
The Plant Journal
Periodical Number
Page range


ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
794 Chetelat, Roger T.
Solanum pimpinellifolium CRISPR/Cas9
Pollen sterility
Basic research
Basic research
795 Chetelat, Roger T.
Solanum pimpinellifolium CRISPR/Cas9
Abundant fruits and seeds by spontaneous self-pollination
Basic research
Basic research