What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

CRISPR/Cas9-mediated viral interference in plants

Typ / Jahr

Journal Article / 2015


Ali, Zahir; Abulfaraj, Aala; Idris, Ali; Ali, Shakila; Tashkandi, Manal; Mahfouz, Magdy M.


BACKGROUND The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species. RESULTS In this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably overexpressing the Cas9 endonuclease, and subsequently challenge these plants with TYLCV. Our data demonstrate that the CRISPR/Cas9 system targeted TYLCV for degradation and introduced mutations at the target sequences. All tested sgRNAs exhibit interference activity, but those targeting the stem-loop sequence within the TYLCV origin of replication in the intergenic region (IR) are the most effective. N. benthamiana plants expressing CRISPR/Cas9 exhibit delayed or reduced accumulation of viral DNA, abolishing or significantly attenuating symptoms of infection. Moreover, this system could simultaneously target multiple DNA viruses. CONCLUSIONS These data establish the efficacy of the CRISPR/Cas9 system for viral interference in plants, thereby extending the utility of this technology and opening the possibility of producing plants resistant to multiple viral infections.

Begomovirus/genetics; CRISPR/Cas9 system; CRISPR-Associated Proteins/metabolism; CRISPR-Cas Systems; Disease Resistance/genetics; Endodeoxyribonucleases/metabolism; Genome, Viral; Plant Diseases/prevention & control/virology; Plant genome engineering; Synthetic site-specific nucleases; Tobacco rattle virus; Tobacco/virology; Tomato yellow leaf curl virus; Viral-mediated genome editing; virus resistance
Genome biology
Periodical Number
Page range


ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
3 Magdy M. Mahfouz
Saudi Arabia
Nicotiana benthamiana CRISPR/Cas9
Delayed or reduced accumulation of viral DNA
Basic research
Basic research