Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Developing a flexible, high-efficiency Agrobacterium -mediated sorghum transformation system with broad application

282

Typ / Jahr

Journal Article / 2018

Autoren

Che, Ping; Anand, Ajith; Wu, Emily; Sander, Jeffry D.; Simon, Marissa K.; Zhu, Weiwei; Sigmund, Amy L.; Zastrow-Hayes, Gina; Miller, Michael; Liu, Donglong; Lawit, Shai J.; Zhao, Zuo-Yu; Albertsen, Marc C.; Jones, Todd J.

Abstract

Sorghum is the fifth most widely planted cereal crop in the world and is commonly cultivated in arid and semi-arid regions such as Africa. Despite its importance as a food source, sorghum genetic improvement through transgenic approaches has been limited because of an inefficient transformation system. Here, we report a ternary vector (also known as cohabitating vector) system using a recently described pVIR accessory plasmid that facilitates efficient Agrobacteriummediated transformation of sorghum. We report regeneration frequencies ranging from 6% to 29% in Tx430 using different selectable markers and single copy, backbone free ‘quality events’ ranging from 45% to 66% of the total events produced. Furthermore, we successfully applied this ternary system to develop transformation protocols for popular but recalcitrant African varieties including Macia, Malisor 84-7 and Tegemeo. In addition, we report the use of this technology to develop the first stable CRISPR/Cas9-mediated gene knockouts in Tx430.

Keywords

Africa sorghum varieties; Agrobacterium; CRISPR/Cas9.; genome modification; sorghum transformation; ternary vector

Periodical

Plant Biotechnol J (Plant Biotechnology Journal)

Periodical Number

Page range

1388–1395

Volume

DOI

10.1111/pbi.12879

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Techniques

ID	Corresponding Author Country	Plant Species	GE Technique Sequence Identifier	Trait Type of Alteration	Progress in Research Key Topic
676	Che, Ping USA	Sorghum bicolor	CRISPR/Cas9 CENH3	Important function in chromosome segregation SDN1	Basic research Basic research