Repositorium
Journal Article / 1999
Zhu, T.; Peterson, D. J.; Tagliani, L.; St. Clair, G.; Baszczynski, C. L.; Bowen, B.
Site-specific heritable mutations in maize genes were engineered by introducing chimeric RNA兾DNA oligonucleotides. Two independent targets within the endogenous maize acetohydroxyacid synthase gene sequence were modified in a site-specific fashion, thereby conferring resistance to either imidazolinone or sulfonylurea herbicides. Similarly, an engineered green fluorescence protein transgene was site-specifically modified in vivo. Expression of the introduced inactive green fluorescence protein was restored, and plants containing the modified transgene were regenerated. Progeny analysis indicated Mendelian transmission of the converted transgene. The efficiency of gene conversion mediated by chimeric oligonucleotides in maize was estimated as 10ⴚ4, which is 1–3 orders of magnitude higher than frequencies reported for gene targeting by homologous recombination in plants. The heritable changes in maize genes engineered by this approach create opportunities for basic studies of plant gene function and agricultural trait manipulation and also provide a system for studying mismatch repair mechanisms in maize.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
657 |
Baszczynski, Chris L. USA |
Zea mays |
Oligonucleotide-directed mutagenesis AHAS |
Herbicide tolerance PM |
Basic research Basic research |
658 |
Baszczynski, Chris L. USA |
Zea mays |
Oligonucleotide-directed mutagenesis GFP |
green fluorescence protein expression PM |
Basic research Basic research |