Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Creation of targeted inversion mutations in plants using an RNA-guided endonuclease

255

Typ / Jahr

Journal Article / 2017

Autoren

Zhang, Congsheng; Liu, Changlin; Weng, Jianfeng; Cheng, Beijiu; Liu, Fang; Li, Xinhai; Xie, Chuanxiao

Abstract

Inversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutations via delivery of a pair of RNA-guided endonucleases (RGENs) of CRISPR/Cas9. The efficiencies of the targeted inversions were 2.6% and 2.2% in the Arabidopsis FLOWERING TIME (AtFT) and TERMINAL FLOWER 1 (AtTFL1) loci, respectively. Thus, we successfully established an approach that can potentially be used to introduce targeted DNA inversions of interest for functional studies and crop improvement.

Keywords

CRISPR/Cas9; genetic improvement; RNA-guided endonuclease; Targeted inversion

Periodical

The Crop Journal

Periodical Number

Page range

83–88

Volume

DOI

10.1016/j.cj.2016.08.001

View Publication

Techniques

ID	Corresponding Author Country	Plant Species	GE Technique Sequence Identifier	Trait Type of Alteration	Progress in Research Key Topic
599	Xie, Chuanxiao China	Arabidopsis thaliana	CRISPR/Cas9 FT	Late flowering/ flowering time/ flowering induction SDN1	Basic research Basic research
600	Xie, Chuanxiao China	Arabidopsis thaliana	CRISPR/Cas9 TFL1	No information SDN1	Basic research Basic research