Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Spontaneous mutation frequency in plants obscures the effect of chimeraplasty

179

Typ / Jahr

Journal Article / 2003

Autoren

Ruiter, René; van den Brande, Ilse; Stals, Ellen; Delauré, Stijn; Cornelissen, Marc; D’Halluin, Kathleen

Abstract

In this study we tested the performance of chimeraplasts, chimeric RNA/DNA oligonucleotides, for the creation of directed changes in chromosomal sequences in tobacco and oilseed rape. As target genes for chimeraplasty, the endogenous als gene and two transgenes, bar and a fusion between egfp and bar, were used. In experiments in which similar numbers of cells were treated with and without chimeraplasts, delivery of chimeraplasts did not lead to increased numbers of herbicide-resistant or egfp fluorescent calli. Sequence analysis of the target genes revealed a range of sequence changes rather than the sequence modification intended through chimeraplast design. Importantly, in both the presence and absence of chimeraplasts, similar types of changes were obtained at similar frequencies. These data suggest that the observed changes at the target codon result primarily from spontaneous mutation.

Keywords

chimeraplasty; endogenous locus (als); spontaneous mutation; transgene locus (egfp-bar and bar)

Periodical

Plant molecular biology

Periodical Number

Page range

675–689

Volume

DOI

10.1023/b:plan.0000019111.96107.01

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Techniques

ID	Corresponding Author Country	Plant Species	GE Technique Sequence Identifier	Trait Type of Alteration	Progress in Research Key Topic
388	D´Halluin Kathleen Belgium	Brassica napus	Oligonucleotide-directed mutagenesis ALS	Herbicide tolerance PM	Market-oriented Herbicide tolerance
389	D´Halluin Kathleen Belgium	Brassica napus	Oligonucleotide-directed mutagenesis bar	Herbicide tolerance PM	Market-oriented Herbicide tolerance
390	D´Halluin Kathleen Belgium	Nicotiana tabacum	Oligonucleotide-directed mutagenesis ALS	herbicide tolerance PM	Basic research Basic research
391	D´Halluin Kathleen Belgium	Nicotiana tabacum	Oligonucleotide-directed mutagenesis bar	herbicide tolerance PM	Basic research Basic research
392	D´Halluin Kathleen Belgium	Nicotiana tabacum	Oligonucleotide-directed mutagenesis egfp	Fluorescence and PM	Basic research Basic research