What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Compact designer TALENs for efficient genome engineering

Typ / Jahr

Journal Article / 2013


Beurdeley, Marine; Bietz, Fabian; Li, Jin; Thomas, Severine; Stoddard, Thomas; Juillerat, Alexandre; Zhang, Feng; Voytas, Daniel F.; Duchateau, Philippe; Silva, George H.


Transcription activator-like effector nucleases are readily targetable ‘molecular scissors’ for genome engineering applications. These artificial nucleases offer high specificity coupled with simplicity in design that results from the ability to serially chain transcription activator-like effector repeat arrays to target individual DNA bases. However, these benefits come at the cost of an appreciably large multimeric protein complex, in which DNA cleavage is governed by the nonspecific FokI nuclease domain. Here we report a significant improvement to the standard transcription activator-like effector nuclease architecture by leveraging the partially specific I-TevI catalytic domain to create a new class of monomeric, DNA-cleaving enzymes. In vivo yeast, plant and mammalian cell assays demonstrate that the half-size, single-polypeptide compact transcription activator-like effector nucleases exhibit overall activity and specificity comparable to currently available designer nucleases. In addition, we harness the catalytic mechanism of I-TevI to generate novel compact transcription activator-like effector nuclease-based nicking enzymes that display a greater than 25-fold increase in relative targeted gene correction efficacy.

Animals; Base Sequence; Catalytic Domain; CHO Cells; Cricetinae; Cricetulus; Deoxyribonuclease I/metabolism; Endodeoxyribonucleases/chemistry/metabolism; Endonucleases/chemistry/metabolism; Genome, Fungal/genetics; Molecular Sequence Data; Protein Engineering/methods; Saccharomyces cerevisiae/genetics; tobacco; Trans-Activators/metabolism
Nature communications
Periodical Number
Page range


ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
21 George H. Silva
Nicotiana benthamiana TALENs
Antibiotic resistance
Basic research
Basic research