Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Conferring resistance to geminiviruses with the CRISPR–Cas prokaryotic immune system

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12

Typ / Jahr

Journal Article / 2015

Autoren

Baltes, Nicholas J.; Hummel, Aaron W.; Konecna, Eva; Cegan, Radim; Bruns, Aaron N.; Bisaro, David M.; Voytas, Daniel F.

Abstract

To reduce crop losses due to geminivirus infection, we targeted the bean yellow dwarf virus (BeYDV) genome for destruction with the CRISPR–Cas (clustered, regularly interspaced short palindromic repeats–CRISPR-associated proteins) system. Transient assays using BeYDV-based replicons revealed that CRISPR–Cas reagents introduced mutations within the viral genome and reduced virus copy number. Transgenic plants expressing CRISPR–Cas reagents and challenged with BeYDV had reduced virus load and symptoms, thereby demonstrating a novel strategy for engineering resistance to geminiviruses.

Keywords
Periodical
NPLANTS (Nature Plants)
Periodical Number
10
Page range
1–3
Volume
1
DOI
10.1038/nplants.2015.145
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Techniques

ID Corresponding Author
Country		 Plant Species GE Technique
Sequence Identifier		 Trait
Type of Alteration		 Progress in Research
Key Topic
19 Voytas, Daniel F.
USA 		Nicotiana benthamiana CRISPR/Cas9
BeYDV 		Virus resistance
SDN1 		Basic research
Basic research