Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Tomato facultative parthenocarpy results from SlAGAMOUS-LIKE 6 loss of function


Typ / Jahr

Journal Article / 2017

Autoren

Klap, Chen; Yeshayahou, Ester; Bolger, Anthony M.; Arazi, Tzahi; Gupta, Suresh K.; Shabtai, Sara; Usadel, Björn; Salts, Yehiam; Barg, Rivka

Abstract

The extreme sensitivity of the microsporogenesis process to moderately high or low temperatures is a major hindrance for tomato (Solanum lycopersicum) sexual reproduction and hence year-round cropping. Consequently, breeding for parthenocarpy, namely, fertilization-independent fruit set, is considered a valuable goal especially for maintaining sustainable agriculture in the face of global warming. A mutant capable of setting high-quality seedless (parthenocarpic) fruit was found following a screen of EMS-mutagenized tomato population for yielding under heat stress. Next-generation sequencing followed by marker-assisted mapping and CRISPR/Cas9 gene knockout confirmed that a mutation in SlAGAMOUS-LIKE 6 (SlAGL6) was responsible for the parthenocarpic phenotype. The mutant is capable of fruit production under heat stress conditions that severely hamper fertilization-dependent fruit set. Different from other tomato recessive monogenic mutants for parthenocarpy, Slagl6 mutations impose no homeotic changes, the seedless fruits are of normal weight and shape, pollen viability is unaffected, and sexual reproduction capacity is maintained, thus making Slagl6 an attractive gene for facultative parthenocarpy. The characteristics of the analysed mutant combined with the gene's mode of expression imply SlAGL6 as a key regulator of the transition between the state of 'ovary arrest' imposed towards anthesis and the fertilization-triggered fruit set.

Keywords
CRISPR/ Cas9; fruit set; Ovary arrest; SlAGL6; Solyc01g093960; Tomato fruit size
Periodical
Plant biotechnology journal
Periodical Number
5
Page range
634–647
Volume
15
DOI
10.1111/pbi.12662

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
208 Barg, Rivka
Israel
Solanum lycopersicum CRISPR/Cas9
AGL6
facultative parthenocarpy
SDN1
Market-oriented
Product quality