Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

The cbfs triple mutants reveal the essential functions of CBFs in cold acclimation and allow the definition of CBF regulons in Arabidopsis


Typ / Jahr

Journal Article / 2016

Autoren

Jia, Yuxin; Ding, Yanglin; Shi, Yiting; Zhang, Xiaoyan; Gong, Zhizhong; Yang, Shuhua

Abstract

In Arabidopsis, the C-repeat binding factors (CBFs) have been extensively studied as key transcription factors in the cold stress response. However, their exact functions in the cold response remains unclear due to the lack of a null cbf triple mutant. In this study, we used CRISPR/Cas9 technology to mutate CBF1 or CBF1/CBF2 in a cbf3 T-DNA insertion mutant to generate cbf1,3 double and cbf1 cbf2 cbf3 (cbfs) triple mutants. The response of the cbfs triple mutants to chilling stress is impaired. Furthermore, no significant difference in freezing tolerance was observed between the wild-type and the cbf1,3 and cbfs mutants without cold acclimation. However, the cbfs mutants were extremely sensitive to freezing stress after cold acclimation, and freezing sensitivity ranking was cbfs > cbf1,3 > cbf3. RNA-Seq analysis showed that 134 genes were CBF regulated, of which 112 were regulated positively and 22 negatively by CBFs. Our study reveals the essential functions of CBFs in chilling stress response and cold acclimation, as well as defines a set of genes as CBF regulon. It also provides materials for the genetic dissection of components in CBF-dependent cold signaling.

Keywords
Arabidopsis; CBF regulons; chilling response; cold acclimation; C-repeat binding factor (CBF) transcription factors
Periodical
The New phytologist
Periodical Number
2
Page range
345–353
Volume
212
DOI
10.1111/nph.14088

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
174 Yang, Shuhua
China
Arabidopsis thaliana CRISPR/Cas9
CBF1/3
Key transcription factors in the cold stress response
SDN1
Basic research
Basic research
175 Yang, Shuhua
China
Arabidopsis thaliana CRISPR/Cas9
CBF1/2/3
Key transcription factors in the cold stress response
SDN1
Basic research
Basic research