Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Establishing a CRISPR-Cas-like immune system conferring DNA virus resistance in plants


Typ / Jahr

Journal Article / 2015

Autoren

Ji, Xiang; Zhang, Huawei; Zhang, Yi; Wang, Yanpeng; Gao, Caixia

Abstract

CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) is an adaptive immune system in many archaea and bacteria that cleaves foreign DNA on the basis of sequence complementarity. Here, using the geminivirus, beet severe curly top virus (BSCTV), transient assays performed in Nicotiana benthamiana demonstrate that the sgRNA-Cas9 constructs inhibit virus accumulation and introduce mutations at the target sequences. Further, transgenic Arabidopsis and N. benthamiana plants overexpressing sgRNA-Cas9 are highly resistant to virus infection.

Keywords
Periodical
NPLANTS (Nature Plants)
Periodical Number
Page range
15144
Volume
1
DOI
10.1038/nplants.2015.144

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
168 Gao, Caixia
China
Arabidopsis thaliana CRISPR/Cas9
BSCTV
BSCTV Resistenz/ Inhibition of viral DNA accumulation
SDN1
Basic research
Basic research
169 Gao, Caixia
China
Nicotiana benthamiana CRISPR/Cas9
BSCTV
BSCTV Resistenz/ Inhibition of viral DNA accumulation
SDN1
Basic research
Basic research