Repositorium
Establishing a CRISPR-Cas-like immune system conferring DNA virus resistance in plants
bearbeiten
82
Typ / Jahr
Journal Article / 2015
Autoren
Ji, Xiang; Zhang, Huawei; Zhang, Yi; Wang, Yanpeng; Gao, Caixia
Abstract
CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) is an adaptive immune system in many archaea and bacteria that cleaves foreign DNA on the basis of sequence complementarity. Here, using the geminivirus, beet severe curly top virus (BSCTV), transient assays performed in Nicotiana benthamiana demonstrate that the sgRNA-Cas9 constructs inhibit virus accumulation and introduce mutations at the target sequences. Further, transgenic Arabidopsis and N. benthamiana plants overexpressing sgRNA-Cas9 are highly resistant to virus infection.
Keywords
Periodical
NPLANTS (Nature Plants)
Periodical Number
Page range
15144
Volume
1
DOI
10.1038/nplants.2015.144
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
168 |
Gao, Caixia China |
Arabidopsis thaliana |
CRISPR/Cas9 BSCTV |
BSCTV Resistenz/ Inhibition of viral DNA accumulation SDN1 |
Basic research Basic research |
169 |
Gao, Caixia China |
Nicotiana benthamiana |
CRISPR/Cas9 BSCTV |
BSCTV Resistenz/ Inhibition of viral DNA accumulation SDN1 |
Basic research Basic research |