Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Targeted genome modifications in soybean with CRISPR/Cas9


Typ / Jahr

Journal Article / 2015

Autoren

Jacobs, Thomas B.; LaFayette, Peter R.; Schmitz, Robert J.; Parrott, Wayne A.

Abstract

Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms. Here, the CRISPR/ Cas9 system is shown to be effective in soybean by knocking-out a green fluorescent protein (GFP) transgene and modifying nine endogenous loci. Results: Targeted DNA mutations were detected in 95% of 88 hairy-root transgenic events analyzed. Bi-allelic mutations were detected in events transformed with eight of the nine targeting vectors. Small deletions were the most common type of mutation produced, although SNPs and short insertions were also observed. Homoeologous genes were successfully targeted singly and together, demonstrating that CRISPR/Cas9 can both selectively, and generally, target members of gene families. Somatic embryo cultures were also modified to enable the production of plants with heritable mutations, with the frequency of DNA modifications increasing with culture time. A novel cloning strategy and vector system based on In-Fusion® cloning was developed to simplify the production of CRISPR/Cas9 targeting vectors, which should be applicable for targeting any gene in any organism. Conclusions: The CRISPR/Cas9 is a simple, efficient, and highly specific genome editing tool in soybean. Although some vectors are more efficient than others, it is possible to edit duplicated genes relatively easily. The vectors and methods developed here will be useful for the application of CRISPR/Cas9 to soybean and other plant species.

Keywords
CRISPR/Cas9; gene targeting; Genomic engineering; Hairy roots; Plant transformation; soybean
Periodical
BMC biotechnology
Periodical Number
Page range
Volume
15
DOI
10.1186/s12896-015-0131-2

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
159 Jacobs, Thomas B.
USA
Glycine Max CRISPR/Cas9
01gDDM1
No information
SDN1
Basic research
Basic research
160 Jacobs, Thomas B.
USA
Glycine Max CRISPR/Cas9
11gDDM1
No information
SDN1
Basic research
Basic research
161 Jacobs, Thomas B.
USA
Glycine Max CRISPR/Cas9
07g14530
glucosyl-transferase
SDN1
Basic research
Basic research
162 Jacobs, Thomas B.
USA
Glycine Max CRISPR/Cas9
Met1
No information
SDN1
Basic research
Basic research
163 Jacobs, Thomas B.
USA
Glycine Max CRISPR/Cas9
miR1509
No information
SDN1
Basic research
Basic research
164 Jacobs, Thomas B.
USA
Glycine Max CRISPR/Cas9
miR1514
No information
SDN1
Basic research
Basic research
165 Jacobs, Thomas B.
USA
Glycine Max CRISPR/Cas9
GFP
green fluorescence protein expression
SDN1
Basic research
Basic research