Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Transient Expression of Virally Delivered Meganuclease In Planta Generates Inherited Genomic Deletions


Typ / Jahr

Journal Article / 2015

Autoren

Honig, Arik; Marton, Ira; Rosenthal, Michal; Smith, J. Jeff; Nicholson, Michael G.; Jantz, Derek; Zuker, Amir; Vainstein, Alexander

Abstract

The use of sequence-specific nucleases for plant genomic DNA mutagenesis is an exciting and rapidly developing technology (Voytas and Merchant, 2013; Baltes and Voytas, 2014). To date, most mutated plants have been recovered from transgenic plants stably expressing nucleases. However, transient nuclease delivery by plant DNA- and RNA-based viral vectors followed by regeneration of plantlets from modified tissues has emerged as an alternative, efficient strategy in some plant species (Marton et al., 2010; Baltes et al., 2014). Delivery of nucleases by plant RNA viruses is particularly attractive because they typically do not integrate into the plant genome, and therefore the mutated plants are not classified as transgenic (Marton et al., 2013; Voytas and Gao, 2014).

Keywords
Alcohol Oxidoreductases/genetics; Base Sequence; Deoxyribonucleases/metabolism; gene expression; Genome, Plant; Inheritance Patterns/genetics; Molecular Sequence Data; Plant Viruses/metabolism; Sequence Deletion; Tobacco/genetics/virology
Periodical
Molecular plant
Periodical Number
8
Page range
1292–1294
Volume
8
DOI
10.1016/j.molp.2015.04.001

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
141 Honig, Arik
Israel
Nicotiana alata Meganucleases
DFR
visible phenotype of reduced purple pigmentation
SDN1
Basic research
Basic research