Repositorium
Transient Expression of Virally Delivered Meganuclease In Planta Generates Inherited Genomic Deletions
71
Journal Article / 2015
Honig, Arik; Marton, Ira; Rosenthal, Michal; Smith, J. Jeff; Nicholson, Michael G.; Jantz, Derek; Zuker, Amir; Vainstein, Alexander
The use of sequence-specific nucleases for plant genomic DNA mutagenesis is an exciting and rapidly developing technology (Voytas and Merchant, 2013; Baltes and Voytas, 2014). To date, most mutated plants have been recovered from transgenic plants stably expressing nucleases. However, transient nuclease delivery by plant DNA- and RNA-based viral vectors followed by regeneration of plantlets from modified tissues has emerged as an alternative, efficient strategy in some plant species (Marton et al., 2010; Baltes et al., 2014). Delivery of nucleases by plant RNA viruses is particularly attractive because they typically do not integrate into the plant genome, and therefore the mutated plants are not classified as transgenic (Marton et al., 2013; Voytas and Gao, 2014).
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
141 |
Honig, Arik Israel |
Nicotiana alata |
Meganucleases DFR |
visible phenotype of reduced purple pigmentation SDN1 |
Basic research Basic research |