Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Germline-transmitted genome editing in Arabidopsis thaliana Using TAL-effector-nucleases


Typ / Jahr

Journal Article / 2015

Autoren

Forner, Joachim; Pfeiffer, Anne; Langenecker, Tobias; Manavella, Pablo A.; Manavella, Pablo; Lohmann, Jan U.

Abstract

Transcription activator-like effector nucleases (TALENs) are custom-made bi-partite endonucleases that have recently been developed and applied for genome engineering in a wide variety of organisms. However, they have been only scarcely used in plants, especially for germline-modification. Here we report the efficient creation of small, germline-transmitted deletions in Arabidopsis thaliana via TALENs that were delivered by stably integrated transgenes. Using meristem specific promoters to drive expression of two TALEN arms directed at the CLV3 coding sequence, we observed very high phenotype frequencies in the T2 generation. In some instances, full CLV3 loss-of-function was already observed in the T1 generation, suggesting that transgenic delivery of TALENs can cause highly efficient genome modification. In contrast, constitutive TALEN expression in the shoot apical meristem (SAM) did not cause additional phenotypes and genome re-sequencing confirmed little off-target effects, demonstrating exquisite target specificity.

Keywords
article; CLV3 gene; controlled study; gene; gene cassette; gene deletion; gene expression; gene frequency; gene locus; gene targeting; genetic engineering; germline transmitted genome editing; loss of function mutation; meristem; onhuman; phenotype; plant genome; promoter region; sequence analysis; shoot; transcription activator like effector nuclease Arabidopsis thaliana; Transgene; ucleotide sequence; whole genome sequencing
Periodical
PloS one
Periodical Number
3
Page range
Volume
10
DOI
10.1371/journal.pone.0121056

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
118 Lohmann, Jan U.
Germany
Arabidopsis thaliana TALENs
CLV3
Altered siliques
SDN1
Basic research
Basic research