Repositorium
Zeitschriftenaufsatz / 2017
Chilcoat, Doane; Liu, Zhan-Bin; Sander, Jeffry
CRISPR/Cas enables precise improvement of commercially relevant crop species by transgenic and nontransgenic methodologies. We have used CRISPR/Cas with or without DNA repair template in both corn and soybean for a range of applications including enhancing drought tolerance, improving seed oil composition, and endowing herbicide tolerance. Importantly, by pairing CRISPR/Cas technology with recent advances in plant tissue culture, these changes can be introduced directly into commercially relevant genotypes. This powerful combination of technologies enables advanced breeding techniques for introducing natural genetic variations directly into product relevant lines with improved speed and quality compared with traditional breeding methods. Variation generated through such CRISPR/Cas enabled advanced breeding approaches can be indistinguishable from naturally occurring variation and therefore should be readily accessible for commercialization. The precision, reach, and flexibility afforded by CRISPR/Cas promise an important role for genome editing in future crop improvement efforts.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
1271 |
Chilcoat, Doane USA |
Zea mays |
CRISPR/Cas9 WX1 |
Waxy phenotype SDN1 |
Market-oriented Product quality |
1272 |
Chilcoat, Doane USA |
Zea mays |
CRISPR/Cas9 ALS2 |
Herbicide tolerance SDN3 |
Market-oriented Herbicide tolerance |
1273 |
Chilcoat, Doane USA |
Zea mays |
CRISPR/Cas9 ARGOS8 |
Drought tolerance SDN3 |
Market-oriented Abiotic stress tolerance |
1274 |
Chilcoat, Doane USA |
Zea mays |
CRISPR/Cas9 SSI target construct |
No information SDN3 |
Basic research Basic research |
1275 |
Chilcoat, Doane USA |
Glycine max |
CRISPR/Cas9 No information |
No information SDN1 |
Basic research Basic research |
1276 |
Chilcoat, Doane USA |
Glycine max |
CRISPR/Cas9 ALS1 |
Herbicide tolerance SDN3 |
Market-oriented Herbicide tolerance |
1277 |
Chilcoat, Doane USA |
Glycine max |
CRISPR/Cas9 No information |
No information SDN1 |
Basic research Basic research |