What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

The RNA Editing Factor SlORRM4 Is Required for Normal Fruit Ripening in Tomato

Typ / Jahr

Journal Article / 2017


Yang, Yongfang; Zhu, Guoning; Li, Rui; Yan, Shijie; Fu, Daqi; Zhu, Benzhong; Tian, Huiqin; Luo, Yunbo; Zhu, Hongliang


RNA editing plays a key posttranscriptional role in gene expression. Existing studies on cytidine-to-uridine RNA editing in plants have focused on maize (Zea mays), rice (Oryza sativa), and Arabidopsis (Arabidopsis thaliana). However, the importance and regulation of RNA editing in several critical agronomic processes are not well understood, a notable example of which is fruit ripening. Here, we analyzed the expression profile of 33 RNA editing factors and identified 11 putative tomato (Solanum lycopersicum) fruit ripening-related factors. A rapid virus-induced gene silencing assay indicated that the organelle RNA recognition motif-containing protein SlORRM4 affected tomato fruit ripening. Knocking out SlORRM4 expression using a clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome editing strategy delayed tomato fruit ripening by lowering respiratory rate and ethylene production. Additionally, the expression of numerous genes associated with fruit ripening and mitochondrial functions changed significantly when SlORRM4 was knocked out. Moreover, the loss of SlORRM4 function significantly reduced RNA editing of many mitochondrial transcripts, leading to low-level expression of some core subunits that are critical for mitochondrial complex assembly (i.e. Nad3, Cytc1, and COX II). Taken together, these results indicate that SlORRM4 is involved in RNA editing of transcripts in ripening fruit that influence mitochondrial function and key aspects of fruit ripening.

CRISPR-Cas Systems; Fruit/physiology; Gene Expression Regulation, Developmental/physiology; Gene Expression Regulation, Plant/physiology; Gene silencing; Lycopersicon esculentum/genetics/physiology; Mitochondria; Mutation; phylogeny; Plant Development/genetics/physiology; Plant Proteins/genetics/metabolism; RNA Editing/genetics/physiology
Plant physiology
Periodical Number
Page range


ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
1267 Zhu, Hongliang
Solanum lycopersicum CRISPR/Cas9
Delayed fruit ripening
Basic research
Basic research