Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Induction of Xa10-like Genes in Rice Cultivar Nipponbare Confers Disease Resistance to Rice Bacterial Blight


Typ / Jahr

Journal Article / 2017

Autoren

Wang, Jun; Tian, Dongsheng; Gu, Keyu; Yang, Xiaobei; Wang, Lanlan; Zeng, Xuan; Yin, Zhongchao

Abstract

Bacterial blight of rice, caused by Xanthomonas oryzae pv. oryzae, is one of the most destructive bacterial diseases throughout the major rice-growing regions in the world. The rice disease resistance (R) gene Xa10 confers race-specific disease resistance to X. oryzae pv. oryzae strains that deliver the corresponding transcription activator-like (TAL) effector AvrXa10. Upon bacterial infection, AvrXa10 binds specifically to the effector binding element in the promoter of the R gene and activates its expression. Xa10 encodes an executor R protein that triggers hypersensitive response and activates disease resistance. 'Nipponbare' rice carries two Xa10-like genes in its genome, of which one is the susceptible allele of the Xa23 gene, a Xa10-like TAL effector-dependent executor R gene isolated recently from 'CBB23' rice. However, the function of the two Xa10-like genes in disease resistance to X. oryzae pv. oryzae strains has not been investigated. Here, we designated the two Xa10-like genes as Xa10-Ni and Xa23-Ni and characterized their function for disease resistance to rice bacterial blight. Both Xa10-Ni and Xa23-Ni provided disease resistance to X. oryzae pv. oryzae strains that deliver the matching artificially designed TAL effectors (dTALE). Transgenic rice plants containing Xa10-Ni and Xa23-Ni under the Xa10 promoter provided specific disease resistance to X. oryzae pv. oryzae strains that deliver AvrXa10. Xa10-Ni and Xa23-Ni knock-out mutants abolished dTALE-dependent disease resistance to X. oryzae pv. oryzae. Heterologous expression of Xa10-Ni and Xa23-Ni in Nicotiana benthamiana triggered cell death. The 19-amino-acid residues at the N-terminal regions of XA10 or XA10-Ni are dispensable for their function in inducing cell death in N. benthamiana and the C-terminal regions of XA10, XA10-Ni, and XA23-Ni are interchangeable among each other without affecting their function. Like XA10, both XA10-Ni and XA23-Ni locate to the endoplasmic reticulum (ER) membrane, show self-interaction, and induce ER Ca2+ depletion in leaf cells of N. benthamiana. The results indicate that Xa10-Ni and Xa23-Ni in Nipponbare encode functional executor R proteins, which induce cell death in both monocotyledonous and dicotyledonous plants and have the potential of being engineered to provide broad-spectrum disease resistance to plant-pathogenic Xanthomonas spp.

Keywords
Amino Acid Sequence; Base Sequence; Calcium/metabolism; Cell Death/genetics; Disease Resistance/genetics; Gene Expression Regulation, Plant; Host-Pathogen Interactions; Oryza/genetics/microbiology; Plant Diseases/genetics/microbiology; Plant Leaves/cytology/genetics/metabolism; Plant Proteins/genetics/metabolism; Protein Isoforms/genetics/metabolism; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Tobacco/cytology/genetics/metabolism; Xanthomonas/physiology
Periodical
Molecular plant-microbe interactions : MPMI
Periodical Number
6
Page range
466–477
Volume
30
DOI
10.1094/MPMI-11-16-0229-R

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
1253 Yin, Zhongchao
Republic of Singapore
Oryza sativa CRISPR/Cas9
Xa10-Ni
Resistance to Rice bacterial blight
SDN1
Market-oriented
Biotic stress tolerance
1254 Yin, Zhongchao
Republic of Singapore
Oryza sativa CRISPR/Cas9
Xa23-Ni
Resistance to Rice bacterial blight
SDN1
Market-oriented
Biotic stress tolerance
1255 Yin, Zhongchao
Republic of Singapore
Oryza sativa TALENs
Xa10-Ni
Resistance to Rice bacterial blight
SDN1
Market-oriented
Biotic stress tolerance
1256 Yin, Zhongchao
Republic of Singapore
Oryza sativa TALENs
Xa23-Ni
Resistance to Rice bacterial blight
SDN1
Market-oriented
Biotic stress tolerance