What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

CRISPR/Cas9 editing and expression analysis of LOC_Os10g05490 in rice under cold stress

Typ / Jahr

Journal Article / 2017


Shen, Chunxiu


To explore the expression and cold tolerance function of LOC_Os10g05490 in rice under cold stress, semiquantitative analysis was performed for Dongxiang wild rice and 93-11. The results showed that expression level of LOC_Os10g05490 in 93-11 was the same before and after cold stress, but it was down regulated in Dongxiang wild rice after cold stress. This was consistent with RNA-seq data and further study indicated that this gene might be associated with cold tolerance in Dongxiang wild rice. Plant expression vectors with CRISPR/Cas9 genome editing technique were subsequently constructed to target the LOC_Os10g05490 locus, and were transformed into the japonica rice variety TP309. A total of 26 transgenic positive plants were obtained. This study may lay the foundation for subsequent screening of mutant plants and identification of genes associated with cold tolerance in rice.

cold stress; CRISPR/Cas9 genome editing technique; Dongxiang wild rice; RNA-Seq
Periodical Number
Page range


ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
1245 Shen, Chunxiu
Oryza sativa CRISPR/Cas9
Cold tolerance
Basic research
Basic research