Repositorium
CRISPR/Cas9 editing and expression analysis of LOC_Os10g05490 in rice under cold stress
503
Journal Article / 2017
Shen, Chunxiu
To explore the expression and cold tolerance function of LOC_Os10g05490 in rice under cold stress, semiquantitative analysis was performed for Dongxiang wild rice and 93-11. The results showed that expression level of LOC_Os10g05490 in 93-11 was the same before and after cold stress, but it was down regulated in Dongxiang wild rice after cold stress. This was consistent with RNA-seq data and further study indicated that this gene might be associated with cold tolerance in Dongxiang wild rice. Plant expression vectors with CRISPR/Cas9 genome editing technique were subsequently constructed to target the LOC_Os10g05490 locus, and were transformed into the japonica rice variety TP309. A total of 26 transgenic positive plants were obtained. This study may lay the foundation for subsequent screening of mutant plants and identification of genes associated with cold tolerance in rice.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
1245 |
Shen, Chunxiu China |
Oryza sativa |
CRISPR/Cas9 Os10g05490 |
Cold tolerance SDN1 |
Basic research Basic research |