CRISPR/CAS9-mediated Editing of the Fragrant Gene Badh2 in Rice
Journal Article / 2017
SHAO Gaoneng, XIE Lihong, JIAO Guiai, WEI Xiangjin, SHENG Zhonghua, TANG Shaoqing, HU Peisong
【Objective】Fragrant rice, which is favored because of its quality characteristics of faint scent and tastiness, is a special rice type. Fragrance in rice is mainly controlled by the gene encoding betaine aldehyde dehydrogenase on chromosome 8.【Method】The fragrant gene Badh2 in Zhonghua 11 was edited by CRISPR/CAS9.【Result】Twenty T1 individuals derived from T0 generation were genotyped. One plant, which contains an additional T base in the first exon of Badh2 without the vector skeleton, was finally produced. qRT-PCR result suggested that Badh2 RNA level was decreased in the mutant. Compared with the wild type, the mutant increased 2-acetyl-1-pyrroline content by the GC-MS method. Furthermore, two rice yield-related traits including tiller numbers and seed-setting rate showed significant difference at the 0.05 level among five traits related to yield and three traits related to cooking and eating quality.【Conclusion】We succeed editing the Badh2 by CRISPR-CAS9 technical in rice, and it would provide abundant theoretical guidance and accelerate the breeding process of fragrant rice.
|Plant Species||GE Technique
Type of Alteration
|Progress in Research
Tang, Shaoqing; Hu, Peisong