What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Re-evaluation of the rin mutation and the role of RIN in the induction of tomato ripening

Typ / Jahr

Journal Article / 2017


Ito, Yasuhiro; Nishizawa-Yokoi, Ayako; Endo, Masaki; Mikami, Masafumi; Shima, Yoko; Nakamura, Nobutaka; Kotake-Nara, Eiichi; Kawasaki, Susumu; Toki, Seiichi


Tomato (Solanum lycopersicum) rin mutants completely fail to ripen: they do not produce red pigmentation, soften or induce an ethylene burst. Therefore, RIN has long been believed to function as a major regulator that is essential for the induction of ripening. Here, we provide evidence contradicting this concept of RIN function, showing induction of fruit ripening in the absence of RIN. A CRISPR/Cas9-mediated RIN-knockout mutation did not repress initiation of ripening and the mutant fruits showed moderate red colouring. Moreover, inactivation of the rin mutant allele partially restored the induction of ripening. Therefore, RIN is not required for the initiation of ripening and rin is not a null mutation, but rather is a gain-of-function mutation that produces a protein that actively represses ripening. Since the discovery of the rin mutant a half-century ago, many models have depicted RIN as indispensable for the induction of ripening; these models should be reconsidered in light of these results.

Alleles; Fruit/genetics/growth & development; Gene Expression Regulation, Plant; Gene Knockout Techniques; Genes, Plant; Genes, Recessive; Lycopersicon esculentum/genetics/growth & development; MADS Domain Proteins/genetics/physiology; Mutation; Plant Proteins/genetics/physiology; Promoter Regions, Genetic; Protein Binding
Nature plants
Periodical Number
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ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
1213 Ito, Yasuhiro
Solanum lycopersicum CRISPR/Cas9
Fruit ripening
Basic research
Basic research