What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Generation of the Mutations for OsYUCCA1 in Rice Using CRISPR/Cas9 Approach

Typ / Jahr

Journal Article / 2017


He Xianchang; Huang Guoqiang; Wang Daoyang; Chang Shuwei; Zhang Dabing


To investigate the role of auxin in model monocot crop rice (Oryza sativa), we constructed two vectors targeting to mutate rice auxin synthetic gene OsYUCCA1 via CRISPR/Cas9 system. The two targeted site sequences of OsYUCCA1 were synthesized and inserted into the vector called hspCas9 and then linked to the binary vector of pCAMBIA1300 . The resultant plasmids were transferred into rice through Agrobacteriummediated transformation. Three types of mutations were identified from 78 transgenic plants, including 26 homologous plants with A or T insertion respectively at 260th base on the exon and 22 heterogeneous plants with A insertion at 447th, C replaced by T at 444th and G replaced by C at 445th on the exon. Moreover, the three types of mutations of OsYUCCA1 caused a premature stop and functional loss protein in the homologous mutants. The knockout mutants of OsYUCCA1 created by CRISPR/Cas9 provides the theoretical basis for further study on the role ofauxin in rice.

auxin; CRISPR/Cas9; OsYUCCA1; rice; Technology ofgene editing
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ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
1212 Zhang, Dabing
Oryza sativa CRISPR/Cas9
No information
Basic research
Basic research