Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

ZmCCT9 enhances maize adaptation to higher latitudes


Typ / Jahr

Journal Article / 2018

Autoren

Huang, Cheng; Sun, Huayue; Xu, Dingyi; Chen, Qiuyue; Liang, Yameng; Wang, Xufeng; Xu, Guanghui; Tian, Jinge; Wang, Chenglong; Li, Dan; Wu, Lishuan; Yang, Xiaohong; Jin, Weiwei; Doebley, John F.; Tian, Feng

Abstract

From its tropical origin in southwestern Mexico, maize spread over a wide latitudinal cline in the Americas. This feat defies the rule that crops are inhibited from spreading easily across latitudes. How the widespread latitudinal adaptation of maize was accomplished is largely unknown. Through positional cloning and association mapping, we resolved a flowering-time quantitative trait locus to a Harbinger-like transposable element positioned 57 kb upstream of a CCT transcription factor (ZmCCT9). The Harbinger-like element acts in cis to repress ZmCCT9 expression to promote flowering under long days. Knockout of ZmCCT9 by CRISPR/Cas9 causes early flowering under long days. ZmCCT9 is diurnally regulated and negatively regulates the expression of the florigen ZCN8, thereby resulting in late flowering under long days. Population genetics analyses revealed that the Harbinger-like transposon insertion at ZmCCT9 and the CACTA-like transposon insertion at another CCT paralog, ZmCCT10, arose sequentially following domestication and were targeted by selection for maize adaptation to higher latitudes. Our findings help explain how the dynamic maize genome with abundant transposon activity enabled maize to adapt over 90° of latitude during the pre-Columbian era.

Keywords
Domestication; flowering time; maize adaptation; Transposable element; ZmCCT9
Periodical
Proceedings of the National Academy of Sciences of the United States of America
Periodical Number
2
Page range
Volume
115
DOI
10.1073/pnas.1718058115

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
986 Doebley, John F.
USA
Zea mays CRISPR/Cas9
CCT9
Early flowering under long days
SDN1
Market-oriented
Agronomic value