Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

OsGIF1 Positively Regulates the Sizes of Stems, Leaves, and Grains in Rice


Typ / Jahr

Journal Article / 2017

Autoren

He, Zhongshan; Zeng, Jing; Ren, Yun; Chen, Dan; Li, Wenjie; Gao, Fengyan; Cao, Ye; Luo, Tao; Yuan, Guoqiang; Wu, Xianghong; Liang, Yueyang; Deng, Qiming; Wang, Shiquan; Zheng, Aiping; Zhu, Jun; Liu, Huainian; Wang, Lingxia; Li, Ping; Li, Shuangcheng

Abstract

Growth-regulating factor (GRF) interacting factors (GIFs) are involved in several developmental processes in Arabidopsis. We previously showed that upregulation of OsGIF1 expression improves rice grain size. However, whether OsGIF1 is involved in other developmental processes remains unclear. Here, we report pleiotropic effects of OsGIF1 on rice organ size regulation. Overexpression and functional knock-out via a CRISPR/Cas9 strategy revealed that OsGIF1 not only positively regulates the sizes of rice leaf, stem, and grain but also influences rice reproduction. Expression profiles based on both qRT-PCR and GUS (β-glucuronidase) histochemical staining suggested that OsGIF1 is differentially expressed across various rice tissues, consistent with its roles in regulating the development of multiple rice organs. Additionally, we found that OsGIF1-GFP localized preferentially in the nucleus, which supports its proposed role as a transcriptional cofactor. Further histological analysis suggested that OsGIF1 affected rice organ size possibly by regulating cell size. Our results suggest that OsGIF1 plays important roles in vegetative and reproductive developmental processes, with important implications for rice breeding.

Keywords
cell size; knock-out; organ size; OsGIF1; rice
Periodical
Frontiers in plant science
Periodical Number
Page range
1730
Volume
8
DOI
10.3389/fpls.2017.01730

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
978 Li, Ping; Li, Shuangcheng
China
Oryza sativa CRISPR/Cas9
GIF1
Reduction of plant height,decreased stem, leave, and grain size
SDN1
Basic research
Basic research