Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Efficient targeted DNA editing and replacement in Chlamydomonas reinhardtii using Cpf1 ribonucleoproteins and single-stranded DNA


Typ / Jahr

Journal Article / 2017

Autoren

Ferenczi, Aron; Pyott, Douglas Euan; Xipnitou, Andromachi; Molnar, Attila

Abstract

The green alga Chlamydomonas reinhardtii is an invaluable reference organism to research fields including algal, plant, and ciliary biology. Accordingly, decades-long standing inefficiencies in targeted nuclear gene editing broadly hinder Chlamydomonas research. Here we report that single-step codelivery of CRISPR/Cpf1 ribonucleoproteins with single-stranded DNA repair templates results in precise and targeted DNA replacement with as much as ∼10% efficiency in C. reinhardtii We demonstrate its use in transgene- and selection-free generation of sequence-specific mutations and epitope tagging at an endogenous locus. As the direct delivery of gene-editing reagents bypasses the use of transgenes, this method is potentially applicable to a wider range of species without the need to develop methods for stable transformation.

Keywords
Chlamydomonas reinhardtii; CRISPR/Cpf1; editing; RNP; ssODN
Periodical
Proceedings of the National Academy of Sciences of the United States of America
Periodical Number
51
Page range
13567–13572
Volume
114
DOI
10.1073/pnas.1710597114

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
967 Molnar, Attila
United Kingdom
Chlamydomonas reinhardtii CRISPR/Cpf1
FKB12
No information
SDN1
Basic research
Basic research
968 Molnar, Attila
United Kingdom
Chlamydomonas reinhardtii CRISPR/Cpf1
FKB12
No information
SDN2
Basic research
Basic research
969 Molnar, Attila
United Kingdom
Chlamydomonas reinhardtii CRISPR/Cpf1
FTSY
bright green phenotype
SDN2
Basic research
Basic research
970 Molnar, Attila
United Kingdom
Chlamydomonas reinhardtii CRISPR/Cpf1
SRP43
bright green phenotype
SDN2
Basic research
Basic research
971 Molnar, Attila
United Kingdom
Chlamydomonas reinhardtii CRISPR/Cpf1
PHT7
No information
SDN2
Basic research
Basic research
972 Molnar, Attila
United Kingdom
Nicotiana benthamiana CRISPR/Cpf1
GFP
Green fluorescence
SDN1
Basic research
Basic research