Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator


Typ / Jahr

Journal Article / 2017

Autoren

Ajjawi, Imad; Verruto, John; Aqui, Moena; Soriaga, Leah B.; Coppersmith, Jennifer; Kwok, Kathleen; Peach, Luke; Orchard, Elizabeth; Kalb, Ryan; Xu, Weidong; Carlson, Tom J.; Francis, Kristie; Konigsfeld, Katie; Bartalis, Judit; Schultz, Andrew; Lambert, William; Schwartz, Ariel S.; Brown, Robert; Moellering, Eric R.

Abstract

Lipid production in the industrial microalga Nannochloropsis gaditana exceeds that of model algal species and can be maximized by nutrient starvation in batch culture. However, starvation halts growth, thereby decreasing productivity. Efforts to engineer N. gaditana strains that can accumulate biomass and overproduce lipids have previously met with little success. We identified 20 transcription factors as putative negative regulators of lipid production by using RNA-seq analysis of N. gaditana during nitrogen deprivation. Application of a CRISPR-Cas9 reverse-genetics pipeline enabled insertional mutagenesis of 18 of these 20 transcription factors. Knocking out a homolog of fungal Zn(II)2Cys6-encoding genes improved partitioning of total carbon to lipids from 20% (wild type) to 40-55% (mutant) in nutrient-replete conditions. Knockout mutants grew poorly, but attenuation of Zn(II)2Cys6 expression yielded strains producing twice as much lipid (∼5.0 g m-2 d-1) as that in the wild type (∼2.5 g m-2 d-1) under semicontinuous growth conditions and had little effect on growth.

Keywords
Periodical
Nature biotechnology
Periodical Number
7
Page range
647–652
Volume
35
DOI
10.1038/nbt.3865

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
928 Ajjawi, Imad; Moellering Eric. M
USA
Nannochloropsis gaditana CRISPR/Cas9
Zn2CYS
Producing the double amount of lipids
SDN3
Basic research
Basic research