What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

OsSLA4 encodes a pentatricopeptide repeat protein essential for early chloroplast development and seedling growth in rice

Typ / Jahr

Journal Article / 2018


Wang, Zhong-wei; Lv, Jun; Xie, Shu-zhang; Zhang, Yu; Qiu, Zhen-nan; Chen, Ping; Cui, Yong-tao; Niu, Yao-fang; Hu, Shi-kai; Jiang, Hong-zhen; Ge, Sheng-zhen; Trinh, HaiPhuong; Lei, Kai-rong; Bai, Wen-qin; Zhang, Yi; Guo, Long-biao; Ren, De-yong


In land plants, chloroplast transcripts undergo post-transcriptional modifications, including splicing, editing, trimming, etc., before translation, and a set of nuclearencoded proteins regulate this essential step. In this study, we characterized a rice (Oryza sativa) seedling-lethal albino mutant sla4 from the progeny of tissue culture plants of the japonica cultivar Zhonghua 11. The sla4 mutant exhibited an albino phenotype from germination through the thirdleaf stage, and then gradually died. The sla4 mutants lacked photosynthetic pigments and had severe defects in photosynthesis and early chloroplast development. Map-based cloning showed that a 13-bp deletion in the coding region of OsSLA4 on chromosome 7 resulted in the albino phenotype and albino mutants were also generated by knockingout OsSLA4 in wild type with the CRISPR/Cas9 system. OsSLA4 encodes a chloroplast-localized pentatricopeptide repeat (PPR) protein with 15 PPR motifs and an atypical DYW-like motif. Loss-of-function of OsSLA4 resulted in severe defects in the intron splicing of atpF, ndhA, petB, rpl2, rpl16, rps12-2, and trnG, as well as a significant reduction in the transcript levels of chloroplast ribosomal RNAs and some chloroplast development- and photosynthesisrelated genes. These results indicate that OsSLA4 is indispensable for early chloroplast development and seedling growth in rice, most likely acting by influencing the intron splicing of multiple chloroplast group II introns.

Chloroplast development; Oryza sativa · Seedling-lethal albino ·; Pentatricopeptide repeat protein · Intron splicing ·
Plant Growth Regul (Plant Growth Regulation)
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ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
842 Zhang, Yi; Guo, Long-biao; Ren, De-yong
Oryza sativa CRISPR/Cas9
Albino phenotype
Basic research
Basic research