Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Inheritance of co-edited genes by CRISPR-based targeted nucleotide substitutions in rice


Typ / Jahr

Journal Article / 2018

Autoren

Shimatani, Zenpei; Fujikura, Ushio; Ishii, Hisaki; Matsui, Yusuke; Suzuki, Minoru; Ueke, Yuki; Taoka, Ken-ichiro; Terada, Rie; Nishida, Keiji; Kondo, Akihiko

Abstract

The CRISPR/Cas9 system is a revolutionary genome-editing tool for directed gene editing in various organisms. Cas9 variants can be applied as molecular homing devices when combined with various functional effectors such as transcriptional activators or DNA modification enzymes. Target-AID is a synthetic complex of nuclease deficient Cas9 fused to an activation-induced cytidine deaminase (AID) that enables targeted nucleotide substitution (C to T or G to A). We previously demonstrated that the introduction of desired point mutations into target genes by Target-AID confers herbicide tolerance to rice callus. Inheritance of the introduced mutations, as well as the removal of transgenes, are key issues that must be addressed in order to fully develop Target-AID as a plant breeding technique. Here we report the transmission of such mutations from the callus to regenerants and their progenies, leading to a generation of selectable marker-free (SMF) herbicide tolerant rice plants with simultaneous multiplex nucleotide substitutions. These findings demonstrate that Target-AID can be developed into novel plant breeding technology which enables improvement of multiplex traits at one time in combination with sophisticated targeted base editing with the simplicity and versatility of CRISPR/Cas9 system.

Keywords
Acetolactate synthase (ALS); Activation-induced cytidine deaminase (AID); herbicide tolerance; rice; Selectable marker-free (SMF); Target-AID; Targeted nucleotide editing
Periodical
Plant physiology and biochemistry : PPB
Periodical Number
Page range
Volume
DOI
10.1016/j.plaphy.2018.04.028

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
814 Nishida, Keji; Kondo, Akihiko
Japan
Oryza sativa Base-editing
FTIP1e
No information
BE
Basic research
Basic research
815 Nishida, Keji; Kondo, Akihiko
Japan
Oryza sativa Base-editing
ALS
Herbicide tolerance
BE
Market-oriented
Herbicide tolerance