Repositorium
Journal Article / 2017
Sánchez-León, Susana; Gil-Humanes, Javier; Ozuna, Carmen V.; Giménez, María J.; Sousa, Carolina; Voytas, Daniel F.; Barro, Francisco
Coeliac disease is an autoimmune disorder triggered in genetically predisposed individuals by the ingestion of gluten proteins from wheat, barley and rye. The α-gliadin gene family of wheat contains four highly stimulatory peptides, of which the 33-mer is the main immunodominant peptide in patients with coeliac. We designed two sgRNAs to target a conserved region adjacent to the coding sequence for the 33-mer in the α-gliadin genes. Twenty-one mutant lines were generated, all showing strong reduction in α-gliadins. Up to 35 different genes were mutated in one of the lines of the 45 different genes identified in the wild type, while immunoreactivity was reduced by 85%. Transgene-free lines were identified, and no off-target mutations have been detected in any of the potential targets. The low-gluten, transgene-free wheat lines described here could be used to produce low-gluten foodstuff and serve as source material to introgress this trait into elite wheat varieties.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
810 |
Gil-Humanes, Javier; Barro Francisco Spain; USA |
Triticum aestivum |
CRISPR/Cas9 a-gliadin |
Reduced gluten content SDN1 |
Market-oriented Product quality |
811 |
Gil-Humanes, Javier; Barro Francisco Spain; USA |
Triticum durum |
CRISPR/Cas9 a-gliadin |
Reduced gluten content SDN1 |
Market-oriented Product quality |