What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Zinc finger nuclease-mediated precision genome editing of an endogenous gene in hexaploid bread wheat ( Triticum aestivum ) using a DNA repair template

Typ / Jahr

Journal Article / 2018


Ran, Yidong; Patron, Nicola; Kay, Pippa; Wong, Debbie; Buchanan, Margaret; Cao, Ying-Ying; Sawbridge, Tim; Davies, John P.; Mason, John; Webb, Steven R.; Spangenberg, German; Ainley, William M.; Walsh, Terence A.; Hayden, Matthew J.


Sequence-specific nucleases have been used to engineer targeted genome modifications in various plants. While targeted gene knockouts resulting in loss of function have been reported with relatively high rates of success, targeted gene editing using an exogenously supplied DNA repair template and site-specific transgene integration has been more challenging. Here, we report the first application of zinc finger nuclease (ZFN)-mediated, nonhomologous end-joining (NHEJ)-directed editing of a native gene in allohexaploid bread wheat to introduce, via a supplied DNA repair template, a specific single amino acid change into the coding sequence of acetohydroxyacid synthase (AHAS) to confer resistance to imidazolinone herbicides. We recovered edited wheat plants having the targeted amino acid modification in one or more AHAS homoalleles via direct selection for resistance to imazamox, an AHAS-inhibiting imidazolinone herbicide. Using a cotransformation strategy based on chemical selection for an exogenous marker, we achieved a 1.2% recovery rate of edited plants having the desired amino acid change and a 2.9% recovery of plants with targeted mutations at the AHAS locus resulting in a loss-of-function gene knockout. The latter results demonstrate a broadly applicable approach to introduce targeted modifications into native genes for nonselectable traits. All ZFNmediated changes were faithfully transmitted to the next generation.

acetohydroxyacid synthase; end-joining; genome editing; herbicide tolerance; nonhomologous; wheat.; zinc finger nuclease
Plant Biotechnol J (Plant Biotechnology Journal)
Periodical Number
Page range


ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
797 Hayden, Matthew J.
Triticum aestivum Zinc-finger nucleases
Herbicide tolerance
Basic research
Basic research
798 Hayden, Matthew J.
Triticum aestivum Zinc-finger nucleases
Herbicide tolerance
Basic research
Basic research