Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Establishment of CRISPR/Cas9 Genome Editing System Based on Gb U6 Promoters in Cotton ( Gossypium barbadense L.)


Typ / Jahr

Journal Article / 2018

Autoren

LI, Ji-Yang; LEI, Jian-Feng; DAI, Pei-Hong; YAO, Rui; QU, Yan-Ying; CHEN, Quan-Jia; LI, Yue; LIU, Xiao-Dong

Abstract

Abstract: CRISPR/Cas9 genome editing is a powerful tool for genes functional analyses, and the mutation of endogenous genes has been successfully implemented in many organisms using the tool. Two cloned U6 promoter from sea island cotton Xinhai 16 were used to construct CRISPR/Cas9 gene editing vectors with target (GbGGB and GBERA1) DNA fragments from Xinhai 16 respectively. Through PEG method, the core fragments (including GbU6::sgRNA and CAMV35S::Cas9) of the CRISPR/Cas9 gene editing vectors enriched by PCR method were transformed into the cotton protoplast prepared from the embryo callus of Xinhai 16. The mutation of endogenous target genes was successfully detected by a restriction enzyme PCR (RE-PCR) assay of protoplast genome. The cloning and sequencing of the PCR product, showed that the two Cas9-GbU6-sgRNA vectors could both induce targeted mutagenesis. Sequence analysis revealed that most of the mutations were nucleotide substitutions and the few were nucleotide deletion. The results indicate that the CRISPR/Cas9 gene editing vector system based on GbU6 promoter can realize targeted mutagenesis in sea island cotton, which provides an important technical basis for cotton functional genomics research.

Keywords
cotton; CRISPR/Cas9; genome editing; protoplast
Periodical
Acta Agronomica Sinica
Periodical Number
2
Page range
227
Volume
44
DOI
10.3724/sp.j.1006.2018.00227

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
745 Xiao-Dong, Liu
China
Gossypium barbadense CRISPR/Cas9
GGB
No information
SDN1
Basic research
Basic research
746 Xiao-Dong, Liu
China
Gossypium barbadense CRISPR/Cas9
ERA1
No information
SDN1
Basic research
Basic research