Repositorium
Lycopene Is Enriched in Tomato Fruit by CRISPR/Cas9-Mediated Multiplex Genome Editing
318
Journal Article / 2018
Li, Xindi; Wang, Yanning; Chen, Sha; Tian, Huiqin; Fu, Daqi; Zhu, Benzhong; Luo, Yunbo; Zhu, Hongliang
Numerous studies have been focusing on breeding tomato plants with enhanced lycopene accumulation, considering its positive effects of fruits on the visual and functional properties. In this study, we used a bidirectional strategy: promoting the biosynthesis of lycopene, while inhibiting the conversion from lycopene to β- and α-carotene. The accumulation of lycopene was promoted by knocking down some genes associated with the carotenoid metabolic pathway. Finally, five genes were selected to be edited in genome by CRISPR/Cas9 system using Agrobacterium tumefaciens-mediated transformation. Our findings indicated that CRISPR/Cas9 is a site-specific genome editing technology that allows highly efficient target mutagenesis in multiple genes of interest. Surprisingly, the lycopene content in tomato fruit subjected to genome editing was successfully increased to about 5.1-fold. The homozygous mutations were stably transmitted to subsequent generations. Taken together, our results suggest that CRISPR/Cas9 system can be used for significantly improving lycopene content in tomato fruit with advantages such as high efficiency, rare off-target mutations, and stable heredity.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
740 |
Zhu, Hongliang China |
Solanum lycopersicum |
CRISPR/Cas9 SGR1 |
Accumulation of lycopene SDN1 |
Market-oriented Product quality |
741 |
Zhu, Hongliang China |
Solanum lycopersicum |
CRISPR/Cas9 LCY-E |
catalyzing the cyclisation of lycopene SDN1 |
Market-oriented Product quality |
742 |
Zhu, Hongliang China |
Solanum lycopersicum |
CRISPR/Cas9 Blc |
catalyzing the cyclisation of lycopene SDN1 |
Market-oriented Product quality |
743 |
Zhu, Hongliang China |
Solanum lycopersicum |
CRISPR/Cas9 LCY-B1 |
catalyzing the cyclisation of lycopene SDN1 |
Market-oriented Product quality |
744 |
Zhu, Hongliang China |
Solanum lycopersicum |
CRISPR/Cas9 LCY-B2 |
catalyzing the cyclisation of lycopene SDN1 |
Market-oriented Product quality |