Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

HRM-facilitated rapid identification and genotyping of mutations induced by CRISPR/Cas9 mutagenesis in rice


Typ / Jahr

Journal Article / 2018

Autoren

Li, Shan; Liu, Songmei; Liu, Yanhua; Lu, Haiping; Tan, Yuanyuan; Huang, Jianzhong; Wei, Pengcheng; Shu, Qing Yao

Abstract

Abstract: The clustered regularly interspaced short palindromic repeat (CRISPR)/ CRISPR-associated (Cas) technology has recently emerged as a powerful genomic editing tool with great potential for crop breeding. However, commonly used protocols for screening of CRISPR/Cas9-induced mutations are laborious, timeconsuming, and costly. In the present study we examined the applicability of high resolution melting (HRM) analysis fast screening CRISPR/Cas9-induced mutations in T0 plants and subsequent genotyping of T1 plants derived from single T0 tive analysis demonstrated that HRM analysis could identify mutant T0 typed by HRM analysis using WT parents and T0 populations. Comparaplants carrying various types of mutation, including single nucleotide substitutions and short insertion/deletions, with false positive/negative rates of 0-2.78%. Furthermore, T1 plants could be correctly genoplants as controls. We hence recommend the adoption of HRM analysis in CRISPR/Cas9 mediated genetic studies and breeding in rice and other crop species.

Keywords
bell; Genomic editing; mutation screening; OsLCT1
Periodical
Crop Breed. Appl. Biotechnol. (Crop Breeding and Applied Biotechnology)
Periodical Number
2
Page range
184–191
Volume
18
DOI
10.1590/1984-70332018v18n2a26

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
736 Qing Yao Shu
China
Oryza sativa CRISPR/Cas9
BEL1
herbicide tolerance
SDN1
Basic research
Basic research
737 Qing Yao Shu
China
Oryza sativa CRISPR/Cas9
LCT1
Encodes a cadmium transporter
SDN1
Basic research
Basic research